The role of the medial and central amygdala in stress-induced suppression of pulsatile LH secretion in female rats.
The role of the medial and central amygdala in stress-induced suppression of pulsatile LH secretion in female rats.
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DOI：
10.1210/en.2010-1003
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发表时间：
2011-02
期刊：
影响因子：
4.8
通讯作者：
O'Byrne KT
中科院分区：
文献类型：
--
作者：
Lin Y;Li X;Lupi M;Kinsey-Jones JS;Shao B;Lightman SL;O'Byrne KT
杏仁核的内侧核和中央核分别在心因性和免疫应激诱导的GnRH脉冲发生器抑制中起关键作用。应激通过抑制GnRH和LH的脉动性释放，对生殖功能产生深远的抑制作用。尽管压力源破坏下丘脑GnRH脉冲发生器的机制仍未完全阐明，但许多研究表明杏仁核，特别是其内侧核（MeA）和中央核（CeA），是神经内分泌对压力反应的关键调节剂。在本研究中，我们研究了MeA和CeA在应力诱导的LH脉冲抑制中的作用。切除卵巢的大鼠接受双侧伊博tenic酸或针对MeA或CeA的假病变；通过长期植入的心导管每5 min取血25 μl，持续6 h，用于LH脉冲的测定。基线取样2小时后，大鼠分别暴露于：抑制（1小时）、胰岛素诱导的低血糖（IIH） （0.3 U/kg, iv）或脂多糖（LPS） （25 μg/kg, iv）应激。这种抑制作用，而不是IIH或LPS应激诱导的LH脉冲抑制作用，被MeA损伤明显减弱。相反，CeA损伤能减弱LPS，但不能抑制或抑制IIH应激诱导的LH脉冲。此外，抑制应激后，MeA完整大鼠富gnrh的内侧视前区（mPOA） Fos阳性神经元数量和表达Fos的谷氨酸脱羧酶67神经元比例显著高于损伤大鼠。这些数据表明，MeA和CeA分别在心因性和免疫性应激诱导的GnRH脉冲发生器抑制中起关键作用，并且MeA介导的作用可能涉及mPOA内的γ-氨基丁酸能信号传导。
Medial and central nuclei of the amygdala play a key role in psychogenic and immunological stress-induced suppression of the GnRH pulse generator, respectively. Stress exerts profound inhibitory effects on reproductive function by suppressing the pulsatile release of GnRH and therefore LH. Although the mechanisms by which stressors disrupt the hypothalamic GnRH pulse generator remain to be fully elucidated, numerous studies have implicated the amygdala, especially its medial (MeA) and central nuclei (CeA), as key modulators of the neuroendocrine response to stress. In the present study, we investigated the roles of the MeA and CeA in stress-induced suppression of LH pulses. Ovariectomized rats received bilateral ibotenic acid or sham lesions targeting the MeA or CeA; blood samples (25 μl) were taken via chronically implanted cardiac catheters every 5 min for 6 h for the measurement of LH pulses. After 2 h of baseline sampling, the rats were exposed to either: restraint (1 h), insulin-induced hypoglycemia (IIH) (0.3 U/kg, iv), or lipopolysaccharide (LPS) (25 μg/kg, iv) stress. The restraint but not IIH or LPS stress–induced suppression of LH pulses was markedly attenuated by the MeA lesions. In contrast, CeA lesioning attenuated LPS, but not restraint or IIH stress–induced suppression of LH pulses. Moreover, after restraint stress, the number of Fos-positive neurons and the percentage of glutamic acid decarboxylase67 neurons expressing Fos was significantly greater in the GnRH-rich medial preoptic area (mPOA) of rats with intact, rather than lesioned, MeA. These data indicate that the MeA and CeA play key roles in psychogenic and immunological stress-induced suppression of the GnRH pulse generator, respectively, and the MeA-mediated effect may involve γ-aminobutyric acid ergic signaling within the mPOA.
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影响因子：
2.9
作者：
BELTRAMINO, C;TALEISNIK, S
通讯作者：
TALEISNIK, S
影响因子：
3.4
作者：
Figueiredo, HF;Bruestle, A;Herman, JP
通讯作者：
Herman, JP
影响因子：
2.9
作者：
CARRILLO, AJ;RABII, J;SAWYER, CH
通讯作者：
SAWYER, CH
影响因子：
2.9
作者：
FELDMAN, S;CONFORTI, N;WEIDENFELD, J
通讯作者：
WEIDENFELD, J
影响因子：
--
作者：
BARSELA, ME;CRITCHLOW, V
通讯作者：
CRITCHLOW, V