Preliminary studies on feline coronavirus distribution in naturally and experimentally infected cats.
Preliminary studies on feline coronavirus distribution in naturally and experimentally infected cats.
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DOI：
10.1053/rvsc.1999.0368
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发表时间：
2000-06
影响因子：
2.4
通讯作者：
Belák S
中科院分区：
文献类型：
--
作者：
Kiss I;Kecskeméti S;Tanyi J;Klingeborn B;Belák S
研究了猫冠状病毒在自然感染和实验感染猫中的散毒、组织分布和准种组成。感染仍处于亚临床状态，但大多数动物在整个实验过程中通过粪便排出病毒。通过逆转录聚合酶链反应从皮质、硬脑膜、胰腺、肺、第三眼睑和4例心肌中扩增对应于病毒核衣壳区域的序列。有趣的是，ORF7b病毒区域-一种假定的毒力因子-在较少的器官中被检测到，这提高了该区域在体内病毒复制过程中可能受到缺失影响的可能性。它表明，不同器官之间的病毒准种的组成不同，具有不同功能的基因组区域进行不同的选择过程中，猫冠状病毒的进化过程中应考虑。
The shedding, tissue distribution and quasispecies composition of feline coronaviruses were studied in naturally and experimentally infected cats. The infection remained subclinical, but the majority of the animals shed the virus via faeces throughout the experiment. Sequences corresponding to the viral nucleocapsid region were amplified by reverse-transcription polymerase chain reaction from the cortex, dura mater, pancreas, lungs, third eyelid, and the heart muscle in four cases. Interestingly, the ORF7b viral region – a supposed virulence factor – was detected in fewer organs, raising the possibility that this region can be affected by deletions during virus replication in vivo. It is demonstrated that the composition of the viral quasispecies differs between organs, and that genomic regions with different functions undergo distinct processes of selection, which should be considered during the evolution of feline coronaviruses.
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影响因子：
3.7
作者：
Vennema H;de Groot RJ;Harbour DA;Horzinek MC;Spaan WJ
通讯作者：
Spaan WJ
DOI：
10.1006/smvy.1996.0046
发表时间：
1996-12
期刊：
Seminars in Virology
影响因子：
--
作者：
Lai MM
通讯作者：
Lai MM
影响因子：
1.2
作者：
HOHDATSU, T;OKADA, S;KOYAMA, H
通讯作者：
KOYAMA, H
影响因子：
3.7
作者：
VENNEMA, H;ROSSEN, JWA;ROTTIER, PJM
通讯作者：
ROTTIER, PJM
影响因子：
3.3
作者：
Hohdatsu T;Sasamoto T;Okada S;Koyama H
通讯作者：
Koyama H