Skin-on-a-chip models: General overview and future perspectives.
Skin-on-a-chip models: General overview and future perspectives.
复制标题
芯片上的皮肤模型：概述和未来展望。
DOI：
10.1063/5.0046376
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发表时间：
2021-09
影响因子：
6
通讯作者：
Velasco D
中科院分区：
文献类型：
--
作者：
Risueño I;Valencia L;Jorcano JL;Velasco D
在过去的几年中，在开发和生产用于分析和测试化妆品和药品的体外人体皮肤模型方面取得了一些进展。然而，这些皮肤模型是在静态条件下培养的，这使得它们无法准确地代表正常的人体生理学。最近的兴趣集中在体外3D血管化皮肤模型的生成与动态灌注和微流体设备称为皮肤芯片。这些平台在文献中被广泛描述为组织建模的良好候选者，因为它们能够实现营养物质的更生理性的运输，并允许在毒性、功效和递送方面对候选药物进行高通量和更便宜的评估。在这个角度来看，最近的进展，这些新的平台在动态条件下产生的人体皮肤模型，在体外测试的报告。血管化的人类皮肤等效物（HSEs），转移皮肤芯片（皮肤活检或HSE在芯片中的介绍），和原位皮肤芯片（直接在芯片中的皮肤模型的产生）的进展进行了严格的审查，并讨论了目前使用的方法，在微流控芯片中引入皮肤细胞。最后对该领域的研究现状和发展方向进行了展望。
Over the last few years, several advances have been made toward the development and production of in vitro human skin models for the analysis and testing of cosmetic and pharmaceutical products. However, these skin models are cultured under static conditions that make them unable to accurately represent normal human physiology. Recent interest has focused on the generation of in vitro 3D vascularized skin models with dynamic perfusion and microfluidic devices known as skin-on-a-chip. These platforms have been widely described in the literature as good candidates for tissue modeling, as they enable a more physiological transport of nutrients and permit a high-throughput and less expensive evaluation of drug candidates in terms of toxicity, efficacy, and delivery. In this Perspective, recent advances in these novel platforms for the generation of human skin models under dynamic conditions for in vitro testing are reported. Advances in vascularized human skin equivalents (HSEs), transferred skin-on-a-chip (introduction of a skin biopsy or a HSE in the chip), and in situ skin-on-a-chip (generation of the skin model directly in the chip) are critically reviewed, and currently used methods for the introduction of skin cells in the microfluidic chips are discussed. An outlook on current applications and future directions in this field of research are also presented.
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影响因子：
2.8
作者：
Jeong, Kwang-Hun;Park, Duckshin;Lee, Young-Chul
通讯作者：
Lee, Young-Chul
DOI：
10.1016/j.jiec.2019.09.044
发表时间：
2020-02-25
影响因子：
6.1
作者：
Jeon, Hye Mi;Kim, Kyunghee;Sung, Gun Yong
通讯作者：
Sung, Gun Yong
影响因子：
10
作者：
Abaci, Hasan E.;Guo, Zongyou;Coffman, Abigail;Gillette, Brian;Lee, Wen-han;Sia, Samuel K.;Christiano, Angela M.
通讯作者：
Christiano, Angela M.
影响因子：
3.5
作者：
Alexander FA;Eggert S;Wiest J
通讯作者：
Wiest J
DOI：
10.1073/pnas.0610868104
发表时间：
2007-11-27
影响因子：
11.1
作者：
Huh, Dongeun;Fujioka, Hideki;Takayama, Shuichi
通讯作者：
Takayama, Shuichi