Candidates for the light entrainment pathway to the circadian clock of the Madeira cockroach Rhyparobia maderae
Candidates for the light entrainment pathway to the circadian clock of the Madeira cockroach Rhyparobia maderae
复制标题
马德拉蟑螂 Rhyparobia maderae 生物钟的光夹带途径的候选者
DOI：
10.1007/s00441-013-1757-9
复制
发表时间：
2013
影响因子：
3.6
通讯作者：
Stengl M
中科院分区：
文献类型：
--
作者：
Schendzielorz J;Stengl M
控制马德拉蟑螂运动活动节律的昼夜节律起搏器位于副延髓（AMe）。同侧和对侧复眼可能通过γ-氨基丁酸（GABA）免疫反应（-ir）远端束向AMe提供光输入，该远端束将AMe的肾小球连接到同侧髓质和板层。为了确定可能的光夹带途径，双标记免疫细胞化学进行采用抗体对GABA，肌抑制肽（MIP），allatotropin（AT）和orcokinin（ORC）。虽然所有的抗血清测试，除了抗ORC，显着染色肾小球的AMe，共定位与抗GABA既没有检测到肾小球，也没有在远端道。然而，一个正中神经元，共定位GABA，AT和MIP-免疫反应性似乎连接所有肾小球的AMe的髓质和板。此外，一个远端额腹侧局部神经元与arborizations在所有肾小球的AMe共定位抗AT和抗MIP免疫反应。作为对侧光夹带途径的候选者，一个腹正中神经元和一个腹侧神经元共定位MIP和ORC免疫反应性，通过后连合和前连合投射。两者都在AMe的肾小球间区域分支，其中分支用抗ORC和抗MIP抗血清共标记。MIP在光夹带中的可能作用也得到了Rhyparobia maderae特异性MIP-2注射的支持，该注射在深夜产生了全提前相位响应曲线。未来的实验将挑战我们的假设，GABA-，MIP-和AT-IR神经元提供同侧光夹带所有肾小球，而MIP-和ORC-IR神经元进行对侧光夹带AMe的肾小球间区域，延迟或推进AMe神经元光依赖性。
The circadian pacemaker controlling locomotor activity rhythms in the Madeira cockroach is located at the accessory medulla (AMe). The ipsi- and contralateral compound eyes provide light input to the AMe, possibly via the γ-aminobutyric acid (GABA)-immunoreactive (-ir) distal tract, which connects the glomeruli of the AMe to the ipsilateral medulla and lamina. To identify possible light-entrainment pathways, double-label immunocytochemistry was performed employing antibodies against GABA, myoinhibitory peptide (MIP), allatotropin (AT) and orcokinin (ORC). While all antisera tested, except the anti-ORC, prominently stained the glomeruli of the AMe, colocalization with anti-GABA was detected neither in the glomeruli nor in the distal tract. However, one median neuron that colocalized GABA-, AT- and MIP-immunoreactivity appeared to connect all glomeruli of the AMe to the medulla and lamina. Furthermore, one distal–frontoventral local neuron with arborizations in all glomeruli of the AMe colocalized anti-AT- and anti-MIP immunoreactivity. As candidates for contralateral light entrainment pathways, one ventromedian and one ventral neuron colocalized MIP- and ORC immunoreactivity, projecting via posterior and anterior commissures. Both branched in the interglomerular region of the AMe, where arborizations co-labeled with anti-ORC- and anti-MIP antisera. A possible role for MIP in light entrainment is supported also by injections ofRhyparobia maderae-specific MIP-2, which generated an all-advance phase-response curve late at night. Future experiments will challenge our hypothesis that GABA-, MIP- and AT-ir neurons provide ipsilateral light entrainment to all glomeruli, while MIP- and ORC-ir neurons carry contralateral light entrainment to the AMe’s interglomerular region, either delaying or advancing AMe neurons light-dependently.
登录
查看更多内容
蟑螂 Leucophaea maderae 昼夜节律系统的神经组织
影响因子：
2.8
作者：
U. Homberg;Thomas Reischig;M. Stengl
通讯作者：
M. Stengl
影响因子：
1.5
作者：
Aguilar, Ruth;Maestro, Jose L.;Belles, Xavier
通讯作者：
Belles, Xavier
检查 FMRFamide 相关肽在蟑螂 Leucophaea maderae 生物钟中的作用
影响因子：
3.6
作者：
Sandra Soehler;S. Neupert;R. Predel;M. Stengl
通讯作者：
M. Stengl
辅助髓质异位移植可恢复心律失常蟑螂（Leucophaea maderae）的昼夜节律
影响因子：
2.8
作者：
Thomas Reischig;M. Stengl
通讯作者：
M. Stengl
在蟑螂 Leucophaea maderae 的标准化脑图谱中实施色素分散因子免疫反应神经元
DOI：
--
发表时间：
2010
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Hongying Wei;Basil el Jundi;U. Homberg;M. Stengl
通讯作者：
M. Stengl