Structural and cellular features in metaphyseal and diaphyseal periosteum of osteoporotic rats.
Structural and cellular features in metaphyseal and diaphyseal periosteum of osteoporotic rats.
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DOI：
10.1007/s10735-010-9261-y
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发表时间：
2010-02
影响因子：
3.2
通讯作者：
Xiao, Yin
中科院分区：
文献类型：
--
作者：
Fan, Wei;Bouwense, Stefan A. W.;Crawford, Ross;Xiao, Yin
尽管骨膜在骨质疏松症的发病机制和治疗中发挥着重要的生理作用，但人们对骨质疏松症中骨膜的结构和细胞特征知之甚少。为了研究骨质疏松大鼠骨干和干骺端骨膜的结构和细胞差异，收集骨质疏松和正常雌性Lewis大鼠的右股骨样本，并用苏木精和伊红、抗酒石酸酸性磷酸酶（TRAP）、碱性磷酸酶（ALP）、血管内皮生长因子（VEGF）、血管性血友病（vWF）、酪氨酸抗体或染色试剂盒对组织切片进行染色。羟化酶（TH）和降钙素基因相关肽（CGRP）。结果显示，与正常大鼠相比，骨质疏松大鼠的干骺端骨膜形成层更厚、细胞更多（P < 0.001）。骨质疏松大鼠干骺端骨膜形成层中骨吸收坑中TRAP+破骨细胞数量、VEGF+细胞数量及血管化程度均较高（P<0.05），而两组ALP+细胞数量无显着性差异。 TH染色鉴定的交感神经纤维主要位于骨质疏松大鼠干骺端骨膜形成层。两组间CGRP表达量无明显差异。总之，骨膜可能在骨质疏松大鼠皮质骨吸收中发挥重要作用，且这一病理过程可能受到交感神经系统的调节。
Despite the important physiological role of periosteum in the pathogenesis and treatment of osteoporosis, little is known about the structural and cellular characteristics of periosteum in osteoporosis. To study the structural and cellular differences in both diaphyseal and metaphyseal periosteum of osteoporotic rats, samples from the right femur of osteoporotic and normal female Lewis rats were collected and tissue sections were stained with hematoxylin and eosin, antibodies or staining kit against tartrate resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), vascular endothelial growth factor (VEGF), von Willebrand (vWF), tyrosine hydroxylase (TH) and calcitonin gene-related peptide (CGRP). The results showed that the osteoporotic rats had much thicker and more cellular cambial layer of metaphyseal periosteum compared with other periosteal areas and normal rats (P < 0.001). The number of TRAP+ osteoclasts in bone resorption pits, VEGF+ cells and the degree of vascularization were found to be greater in the cambial layer of metaphyseal periosteum of osteoporotic rats (P < 0.05), while no significant difference was detected in the number of ALP+ cells between the two groups. Sympathetic nerve fibers identified by TH staining were predominantly located in the cambial layer of metaphyseal periosteum of osteoporotic rats. No obvious difference in the expression of CGRP between the two groups was found. In conclusion, periosteum may play an important role in the cortical bone resorption in osteoporotic rats and this pathological process may be regulated by the sympathetic nervous system.
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影响因子：
3.3
作者：
HILL, EL;TURNER, R;ELDE, R
通讯作者：
ELDE, R
影响因子：
2.8
作者：
O'Driscoll, SWM;Saris, DBF;Fitzimmons, JS
通讯作者：
Fitzimmons, JS
影响因子：
4.1
作者：
NAKAHARA, H;BRUDER, SP;CAPLAN, AI
通讯作者：
CAPLAN, AI
影响因子：
9
作者：
Cho, SW;Lim, SH;Kim, BS
通讯作者：
Kim, BS
影响因子：
6.2
作者：
Bliziotes, M;Sibonga, JD;Orwoll, E
通讯作者：
Orwoll, E