Bacterial DNAemia is associated with serum zonulin levels in older subjects.
Bacterial DNAemia is associated with serum zonulin levels in older subjects.
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DOI：
10.1038/s41598-021-90476-0
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发表时间：
2021-05-26
影响因子：
4.6
通讯作者：
Guglielmetti S
中科院分区：
文献类型：
--
作者：
Gargari G;Mantegazza G;Taverniti V;Del Bo' C;Bernardi S;Andres-Lacueva C;González-Domínguez R;Kroon PA;Winterbone MS;Cherubini A;Riso P;Guglielmetti S
血液中细菌数量的增加可能是导致衰老相关疾病发生和进展的一个因素。在此背景下，我们对从疗养院登记的 43 名老年受试者采集的血液样本中的细菌 DNA 进行了量化和分类分析。针对 16S rRNA 基因的定量 PCR 显示，所有样本均含有可检测量的细菌 DNA，其浓度在受试者之间差异很大。相关分析显示，细菌 DNA 血症（以血液中 16S rRNA 基因拷贝浓度表示）与连蛋白（肠道通透性标志物）的血清水平显着相关。通过对从同一受试者采集的第二组血液样本的分析证实了这一结果。 16S rRNA 基因分析显示，血液中检测到的大多数细菌 DNA 属于变形菌门，其中以假单胞菌属为主。还分析了几个对照样品，以评估可能源自试剂和材料的污染物细菌 DNA 的影响。这里报告的数据表明，上皮（以及可能的内皮）细胞层的细胞旁通透性可能在细菌迁移到血流中发挥重要作用。细菌 DNA 血症可能会影响宿主生理学的多个方面，并可能支持老年受试者各种疾病的发展和预后。
The increased presence of bacteria in blood is a plausible contributing factor in the development and progression of aging-associated diseases. In this context, we performed the quantification and the taxonomic profiling of the bacterial DNA in blood samples collected from forty-three older subjects enrolled in a nursing home. Quantitative PCR targeting the 16S rRNA gene revealed that all samples contained detectable amounts of bacterial DNA with a concentration that varied considerably between subjects. Correlation analyses revealed that the bacterial DNAemia (expressed as concentration of 16S rRNA gene copies in blood) significantly associated with the serum levels of zonulin, a marker of intestinal permeability. This result was confirmed by the analysis of a second set of blood samples collected from the same subjects. 16S rRNA gene profiling revealed that most of the bacterial DNA detected in blood was ascribable to the phylum Proteobacteria with a predominance of the genus Pseudomonas. Several control samples were also analyzed to assess the influence of contaminant bacterial DNA potentially originating from reagents and materials. The data reported here suggest that para-cellular permeability of epithelial (and, potentially, endothelial) cell layers may play an important role in bacterial migration into the bloodstream. Bacterial DNAemia is likely to impact on several aspects of host physiology and could underpin the development and prognosis of various diseases in older subjects.
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影响因子：
3.7
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
DOI：
10.1016/j.jaci.2010.10.048
发表时间：
2011-02
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Huang YJ;Nelson CE;Brodie EL;Desantis TZ;Baek MS;Liu J;Woyke T;Allgaier M;Bristow J;Wiener-Kronish JP;Sutherland ER;King TS;Icitovic N;Martin RJ;Calhoun WJ;Castro M;Denlinger LC;Dimango E;Kraft M;Peters SP;Wasserman SI;Wechsler ME;Boushey HA;Lynch SV;National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
影响因子：
3.9
作者：
MA, TY;HOLLANDER, D;KRUGLIAK, P
通讯作者：
KRUGLIAK, P
影响因子：
3.7
作者：
Moreno-Navarrete JM;Sabater M;Ortega F;Ricart W;Fernández-Real JM
通讯作者：
Fernández-Real JM
影响因子：
15.5
作者：
Lim ES;Rodriguez C;Holtz LR
通讯作者：
Holtz LR