Low sensitivity of the formol-ethyl acetate sedimentation concentration technique in low-intensity Schistosoma japonicum infections.
Low sensitivity of the formol-ethyl acetate sedimentation concentration technique in low-intensity Schistosoma japonicum infections.
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DOI：
10.1371/journal.pntd.0000386
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发表时间：
2009
影响因子：
3.8
通讯作者：
Johansen MV
中科院分区：
文献类型：
--
作者：
Lier T;Simonsen GS;Wang T;Lu D;Haukland HH;Vennervald BJ;Johansen MV
日本血吸虫病流行国家在诊断感染者方面面临着越来越大的困难。甲醛-乙酸乙酯沉降浓缩技术是许多临床微生物实验室检测粪便样本中寄生虫的首选方法。它可能比传统上使用的诊断方法更敏感。我们评估了检测低强度S的技术。日本血吸虫感染的106个粪便样本，并使用商业试剂盒，Parasep Midi粪便寄生虫浓缩器。从每个人身上采集一份粪便样本和一份血清样本。作为参考标准，我们使用了血清间接血凝阳性和Kato-Katz厚涂片显微镜检查阳性（来自单个粪便的三个载玻片）和/或孵化试验阳性的人。我们发现沉降技术的敏感性仅为28.6%，特异性为97.4%。这一研究表明，沉降技术在低强度S的诊断中作用不大。日本血吸虫感染，至少在仅检查单个粪便样本时是这样。 日本血吸虫是一种寄生性吸虫，分布于中国、印度尼西亚和菲律宾。人们在防治这种寄生虫方面付出了大量努力，结果是在过去几十年中感染人数大幅下降。一般感染者现在也很少有蠕虫，因此粪便中排出的虫卵很少。这使得通过传统的诊断测试获得正确的诊断变得越来越困难。基于检测粪便中的虫卵的测试可能是假阴性的，检测抗体的测试可能是假阳性的，因为持续存在的抗体或其他蠕虫感染的抗体。因此，需要新的诊断策略。甲醛-乙酸乙酯沉降浓缩是一种通过显微镜检查粪便中虫卵的技术，但据我们所知，尚未对S。山茱萸我们比较了技术，使用一个单一的粪便样本和一个商业化的准备试剂盒，传统上用于流行的国家（加藤-卡茨厚涂片，孵化试验和间接血凝抗体检测）的三个测试。沉降技术检测到的阳性率很低，似乎在诊断低强度S。日本血吸虫感染，与传统使用的测试相比。
The endemic countries are in a diagnostic dilemma concerning Schistosoma japonicum with increasing difficulties in diagnosing the infected individuals. The formol-ethyl acetate sedimentation concentration technique is preferred by many clinical microbiology laboratories for the detection of parasites in stool samples. It is potentially more sensitive than the diagnostic methods traditionally used. We evaluated the technique for detection of low-intensity S. japonicum infections in 106 stool samples from China and used a commercial kit, Parasep Midi Faecal Parasite Concentrator. One stool sample and one serum sample were collected from each person. As reference standard we used persons positive by indirect hemagglutination in serum and positive by Kato-Katz thick smear microscopy (three slides from a single stool), and/or the hatching test. We found the sedimentation technique to have a sensitivity of only 28.6% and specificity of 97.4%. This study indicates that the sedimentation technique has little to offer in the diagnosis of low-intensity S. japonicum infections, at least when only a single stool sample is examined. Schistosoma japonicum is parasitic fluke (worm) found in China, Indonesia and the Philippines. A lot of effort has been put into combating the parasite, and the result has been a large drop in the number of infected people over the last decades. The average infected person also now has few worms, and hence excretes few eggs in stool. This has made it increasingly difficult to get a correct diagnosis by the diagnostic tests traditionally used. Tests based on detecting eggs in stool can be false-negative and tests detecting antibodies can be false-positive due to persisting antibodies or antibodies from other worm infections. Hence there is a need for new diagnostic strategies. Formol-ethyl acetate sedimentation concentration is a technique for detecting eggs in stool by microscopy, but has not to our knowledge been evaluated for S. japonicum. We compared the technique, using a single stool sample and a commercial preparation kit, with three tests traditionally used in the endemic countries (Kato-Katz thick smear, hatching test and indirect hemagglutination antibody detection). The sedimentation technique detected disappointing few positives and seems not to be an advantage in the diagnosis of low-intensity S. japonicum infection, compared to the traditionally used tests.
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DOI：
10.4269/ajtmh.1998.59.370
发表时间：
1998-09-01
影响因子：
3.3
作者：
Yu, J;De Vlas, SJ;Gryseels, B
通讯作者：
Gryseels, B
影响因子：
3.3
作者：
Ramsan, M;Montresor, A;Savioli, L
通讯作者：
Savioli, L
影响因子：
9.4
作者：
TRUANT, AL;ELLIOTT, SH;SMITH, JH
通讯作者：
SMITH, JH
DOI：
10.4269/ajtmh.2007.76.1138
发表时间：
2007-06-01
影响因子：
3.3
作者：
Zhou, Yi-Biao;Yang, Mei-Xia;Jiang, Qing-Wu
通讯作者：
Jiang, Qing-Wu
影响因子：
0.6
作者：
Brown, M;Bukusuba, J;Whitworth, J
通讯作者：
Whitworth, J