Comparative analysis for immune response of coelomic fluid from coelom and polian vesicle in Apostichopus japonicus to Vibrio splendidus infection.
Comparative analysis for immune response of coelomic fluid from coelom and polian vesicle in Apostichopus japonicus to Vibrio splendidus infection.
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DOI：
10.1016/j.fsirep.2022.100074
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发表时间：
2023-12
影响因子：
--
通讯作者：
Li, Qiang
中科院分区：
文献类型：
--
作者：
Wang, Zhenhui;Fan, Xuyuan;Li, Zhen;Guo, Liyuan;Ren, Yuan;Li, Qiang
海参的保质小泡和体腔充满了体腔液，其中悬浮着多种不同功能的体腔细胞。我们前期的工作表明健康刺参体腔细胞两个位点之间的差异主要体现在亚型比例、非特异性免疫酶活性和一些免疫相关基因表达水平上。然而，病原菌感染后体腔液和球囊泡两个部位的体腔液功能异同仍不清楚。在此，我们研究了华丽弧菌病原体感染体腔和Polian小泡后体腔细胞总密度（TCD）和体腔细胞差异密度（DCD）的变化。灿烂弧菌感染后，体腔和保丽小泡中的TCD在12 h时迅速下降，随后体腔中的TCD呈稳定上升趋势，而保丽小泡中的TCD在感染后24 h（hpi）达到峰值，然后从24 hpi到72 hpi呈现持续下降趋势，随后缓慢升高，直至从72 h恢复到正常水平。 hpi 至 96 hpi。然后测定酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性来评价无细胞体腔液对灿烂弧菌感染的反应。 ACP、AKP和CAT的活性在体腔和多聚体小泡中表现出相似的趋势。多聚体小泡中SOD活性显着升高，而体腔中SOD活性呈下降趋势。最后，检测了灿烂葡萄菌攻击后Aj-MyD88、Aj-IRAK4、Aj-i-Lys、Aj-Rel、Aj-p50、Aj-DMBT1、Aj-CDC、Aj-Rrp15和Aj-纤维蛋白原C等9个免疫相关基因的表达谱。结果表明，所有检测到的基因在体腔和聚体小泡中均显着上调，并且除Aj-MyD88和Aj-DMBT1外，这些基因在两个位点中的表达水平具有相似的趋势。这项研究为体腔液和体腔细胞在Polian小泡和体腔对细菌感染的反应中的差异免疫作用提供了新的见解，并为更好地理解日本刺参的先天免疫反应补充了综合资源。
The polian vesicle and coelom of sea cucumber Apostichopus japonicus were full of coelomic fluid in which many types of coelomocytes with different functions were suspended. Our previous work has indicated the differences of coelomocytes between two sites mainly in subtype proportion, non-specific immune enzymes activities and several immune-related genes expression levels in healthy A. japonicus. However, the functional similarities and differences of coelomic fluid in two sites including the coelom and polian vesicle after pathogenic infection still remain unclear. Here, we investigated the changes of the total coelomocyte density (TCD) and differential coelomocyte density (DCD) after pathogen infection by Vibrio splendidus in coelom and polian vesicle. After infected by V. splendidus, the TCD in the coelom and polian vesicle rapidly declined at 12 h, and then the TCD in the coelom showed a stably ascending trend, while the TCD in the polian vesicle reached a peak at 24 h post infection (hpi), and then showed a continuously decline trend from 24 hpi to 72 hpi followed by a slow elevation until recovering the normal level from 72 hpi to 96 hpi. Then the activities of acidic phosphatase (ACP), alkaline phosphatase (AKP), catalase (CAT) and superoxide dismutase (SOD) were determined to evaluate the response of cell-free coelomic fluid to V. splendidus infection. The activities of ACP, AKP and CAT showed similar trends in the coelom and polian vesicle. The SOD activity significantly increased in the polian vesicle, whereas it exhibited a decreasing trend in the coelom. Finally, the expression profiles of nine immune-related genes including Aj-MyD88, Aj-IRAK4, Aj-i-Lys, Aj-Rel, Aj-p50, Aj-DMBT1, Aj-CDC, Aj-Rrp15 and Aj-Fibrinogen C were detected after V. splendidus challenge. The results suggested all the detected genes were significantly up-regulated both in the coelom and polian vesicle, and the expression levels of these genes in two sites shared similar trends except Aj-MyD88 and Aj-DMBT1. This research provides a new insight into the differentially immune roles of coelomic fluid and coelomocytes in polian vesicle and coelom response to bacterial infections and supplements comprehensive resources for better understanding the innate immune response of A. japonicus.
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DOI：
10.1016/j.cbd.2021.100877
发表时间：
2021-07-12
影响因子：
3
作者：
Guo, Liyuan;Wang, Zhenhui;Li, Qiang
通讯作者：
Li, Qiang
仿刺参体腔液与刺参体腔液中非特异性免疫因子的差异及其开膛后的早期反应
影响因子：
4.7
作者：
Ren, Yuan;Zhang, Jialin;Li, Qiang
通讯作者：
Li, Qiang
从海参 (Apostichopus japonicus) 体腔细胞中鉴定出的免疫相关基因响应 LPS 攻击的表达分析。
影响因子：
5.6
作者：
Dong Y;Sun H;Zhou Z;Yang A;Chen Z;Guan X;Gao S;Wang B;Jiang B;Jiang J
通讯作者：
Jiang J
灿烂弧菌攻击后海参体腔细胞免疫途径相关基因的转录组分析和发现
影响因子：
5.6
作者：
Gao Q;Liao M;Wang Y;Li B;Zhang Z;Rong X;Chen G;Wang L
通讯作者：
Wang L
影响因子：
--
作者：
Chiaramonte, M.;Russo, R.
通讯作者：
Russo, R.