Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy.
Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy.
复制标题
具有多光自动荧光显微镜的头颈癌的无标签成像和组织光学评估。
作者：
由于发现较晚，5年生存率仍然很低，因此努力识别具有高恶性潜力的头颈癌病变对改善患者预后非常重要。光学成像方法可以提供直接的细胞和结构异型性，有助于检测和病理指导。本研究评估了多光子自体荧光显微镜（MPAM）方法在切除肿瘤样本中显示与肿瘤相关的非典型性的能力，而不需要外源性染料或组织切片，显示出与相应组织学的相关性。采用多光子自体荧光显微镜（MPAM）方法进行深度分辨无标记光学成像可能为检查与上皮鳞状细胞癌（SCC）相关的细胞和细胞外异型性提供新的方法。我们评估了MPAM在切除的废弃肿瘤组织中识别头颈部组织细胞和微观结构异型的能力。从口腔底部、舌头和喉部的组织中获得三维图像体积，然后进行组织学处理。用MPAM显微照片评价细胞异型性的定性指标和与核多形性相关的定量指标。统计分析将MPAM终点与每个成像部位的组织学分级相关联。通过MPAM观察到的细胞过度拥挤、分离、异核细胞增多和多核细胞与非典型增生和SCC分级有统计学相关性，但与组织学上良性的区域无关。SCC和非典型增生的核大小变异系数的定量测量在统计学上高于组织学上良性的区域。MPAM还允许识别跨过渡区域的细胞异质性和其他特征，如炎症浸润。在未来，MPAM可能作为传统常规检查和活检的辅助手段，用于肿瘤的无创检测。
Efforts to identify lesions of head and neck cancers with high malignant potential are important to improve patient outcomes, as 5-year survival rates remain low due to late detection. Optical imaging approaches that provide direct cellular and structural atypia could be helpful in detection and pathology guidance. This study evaluates the method of multiphoton autofluorescence microscopy (MPAM) for its ability to reveal atypia associated with neoplasia in resected tumor samples without the need for exogenous dyes or tissue sectioning, showing a correlation with the corresponding histology. Depth-resolved label-free optical imaging by the method of multiphoton autofluorescence microscopy (MPAM) may offer new ways to examine cellular and extracellular atypia associated with epithelial squamous cell carcinoma (SCC). MPAM was evaluated for its ability to identify cellular and microstructural atypia in head and neck tissues from resected discarded tumor tissue. Three-dimensional image volumes were obtained from tissues from the floor of the mouth, tongue, and larynx, and were then processed for histology. MPAM micrographs were evaluated for qualitative metrics of cell atypia and quantitative measures associated with nuclear pleomorphism. Statistical analyses correlated MPAM endpoints with histological grade from each imaged site. Cellular overcrowding, discohesion, anisonucleosis, and multinucleated cells, as observed through MPAM, were found to be statistically associated with dysplasia and SCC grading, but not in histologically benign regions. A quantitative measure of the coefficient of variance in nuclear size in SCC and dysplasia was statistically elevated above histologically benign regions. MPAM also allowed for the identification of cellular heterogeneity across transitional areas and other features, such as inflammatory infiltrates. In the future, MPAM could be evaluated for the non-invasive detection of neoplasia, possibly as an adjunct to traditional conventional examination and biopsy.
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影响因子：
2.8
作者：
Fischer, Frank;Volkiner, Beate;Wepf, Roger
通讯作者：
Wepf, Roger
影响因子：
3.9
作者：
Miller DR;Jarrett JW;Hassan AM;Dunn AK
通讯作者：
Dunn AK
影响因子：
3.4
作者：
Jabbour, Joey M.;Bentley, Julie L.;Maitland, Kristen C.
通讯作者：
Maitland, Kristen C.
影响因子：
3.3
作者：
Amirchaghmaghi, Maryam;Mohtasham, Nooshin;Mozafari, Pegah Mosannen
通讯作者：
Mozafari, Pegah Mosannen
影响因子：
2.4
作者：
Pal R;Edward K;Ma L;Qiu S;Vargas G
通讯作者：
Vargas G