In vivo imaging of brain metabolism activity using a phosphorescent oxygen-sensitive probe.
In vivo imaging of brain metabolism activity using a phosphorescent oxygen-sensitive probe.
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DOI：
10.1016/j.jneumeth.2013.04.005
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发表时间：
2013-06-15
影响因子：
3
通讯作者：
Papkovsky, Dmitri B.
中科院分区：
文献类型：
--
作者：
Tsytsarev, Vassiliy;Arakawa, Hiroyuki;Borisov, Sergei;Pumbo, Elena;Erzurumlu, Reha S.;Papkovsky, Dmitri B.
关键词：
已经采用了几种方法来实时成像的神经活动在体内。我们测试了一种新的细胞穿透磷光氧敏感探针，NanO2-IR，监测周围感觉刺激后新皮层中氧代谢的时空动态。将探针溶液施加到麻醉的小鼠脑的表面;使用MiCAM-02系统进行光学成像。在对侧躯体感觉（"桶"）皮层中提供了一系列胡须刺激，并记录了磷光信号的相关变化。感觉刺激导致桶皮质的活化区域的氧合变化。将氧成像结果与由电压敏感染料RH-1691产生的氧成像结果进行比较。虽然两个探针发出的信号在形状和幅度上不同，但它们都忠实地指示了特定的胡须诱发的皮层活动。因此，NanO2-IR探针可用作活体感觉诱发神经活动的可视化和实时分析的工具。
Several approaches have been adopted for real-time imaging of neural activity in vivo. We tested a new cell-penetrating phosphorescent oxygen-sensitive probe, NanO2-IR, to monitor temporal and spatial dynamics of oxygen metabolism in the neocortex following peripheral sensory stimulation. Probe solution was applied to the surface of anesthetized mouse brain; optical imaging was performed using a MiCAM-02 system. Trains of whisker stimuli were delivered and associated changes in phosphorescent signal were recorded in the contralateral somatosensory (“barrel”) cortex. Sensory stimulation led to changes in oxygenation of activated areas of the barrel cortex. The oxygen imaging results were compared to those produced by the voltage-sensitive dye RH-1691. While the signals emitted by the two probes differed in shape and amplitude, they both faithfully indicated specific whisker evoked cortical activity. Thus, NanO2-IR probe can be used as a tool in visualization and realtime analysis of sensory- evoked neural activity in vivo.
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影响因子：
3
作者：
Chen, Yu;Aguirre, Aaron D.;Ruvinskaya, Lana;Devor, Anna;Boas, David A.;Fujimoto, James G.
通讯作者：
Fujimoto, James G.
影响因子：
7.4
作者：
Borisov, S. M.;Nuss, G.;Klimant, I.
通讯作者：
Klimant, I.
影响因子：
3.5
作者：
Lütcke H;Murayama M;Hahn T;Margolis DJ;Astori S;Zum Alten Borgloh SM;Göbel W;Yang Y;Tang W;Kügler S;Sprengel R;Nagai T;Miyawaki A;Larkum ME;Helmchen F;Hasan MT
通讯作者：
Hasan MT
影响因子：
6.3
作者：
Liao, Lun-De;Lin, Chin-Teng;Chen, You-Yin
通讯作者：
Chen, You-Yin
影响因子：
5.7
作者：
Liao, Lun-De;Li, Meng-Lin;Chen, You-Yin
通讯作者：
Chen, You-Yin