Toxicity of Water-Accommodated Fractions (WAF), Chemically Enhanced WAF (CEWAF) of Oman Crude Oil and Dispersant to Early-Life Stages of Zebrafish (Danio rerio)

Toxicity of Water-Accommodated Fractions (WAF), Chemically Enhanced WAF (CEWAF) of Oman Crude Oil and Dispersant to Early-Life Stages of Zebrafish (Danio rerio)
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阿曼原油和分散剂的水容纳部分 (WAF)、化学增强 WAF (CEWAF) 对斑马鱼 (Danio rerio) 生命早期阶段的毒性

DOI:
10.1007/s00128-018-2413-6
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发表时间:
2018-08
影响因子:
2.7
通讯作者:
Xiong Deqi
Xiong Deqi
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Li Xishan;Ding Guanghui;Xiong Yijun;Ma Xinrui;Fan Youmei;Xiong Deqi

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本研究以斑马鱼(Danio Rerio)为研究对象,比较了原油水化馏分(WAF)和化学强化水化馏分(CEWAF)对斑马鱼(Danio Rerio)早期生命阶段(ELS)的致死和亚致死毒性。结果表明,GM-2分散剂的加入增加了总石油烃(TPHs)和总优先多环芳烃(Σ)的含量。根据Σ多环芳烃的估算,WAF和CEWAF的LC20分别为4.88ug/−1和1.19ug/L−1,表明CEWAF的毒性约为其四倍。暴露于CEWAF后,孵化率明显低于WAF,畸形率明显高于WAF。同时,CEWAF的一般形态评分(GMS)值比WAF低约4倍,这表明CEWAF暴露导致了更显著的发育延迟。结果表明,化学分散剂提高了原油对斑马鱼ELS的毒性,其使用可增加鱼类对原油的暴露。
This study focused on comparing the lethal and sublethal toxicity of water-accommodated fractions (WAF) and chemically enhanced WAF (CEWAF) of crude oil to zebrafish (Danio rerio) on early life stages (ELS). Results showed that the addition of GM-2 dispersant caused an increase in the levels of total petroleum hydrocarbons (TPHs) and total priority polycyclic aromatic hydrocarbons (ΣPAHs). Based on ΣPAHs, the LC20estimates for WAF and CEWAF were 4.88 µg L−1and 1.19 µg L−1, respectively, indicating that CEWAF was approximately four times more toxic. CEWAF exposure caused markedly lower hatching rates and higher malformation frequencies than WAF. Meanwhile, the general morphology score (GMS) values in CEWAF were about fourfold lower than that in WAF, indicating that CEWAF exposure induced more significant developmental delay. The results suggested that chemical dispersant enhanced the toxicity of crude oil to zebrafish on ELS and its application could increase the exposure of fishes to crude oil.
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