Post-mating change in excretion by mated Drosophila melanogaster females is a long-term response that depends on sex peptide and sperm.
Post-mating change in excretion by mated Drosophila melanogaster females is a long-term response that depends on sex peptide and sperm.
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DOI：
10.1016/j.jinsphys.2013.07.001
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发表时间：
2013-10
影响因子：
2.2
通讯作者：
Wolfner MF
中科院分区：
文献类型：
--
作者：
Apger-McGlaughon J;Wolfner MF
果蝇精液蛋白在交配后引起雌性生理和行为的变化。例如，精液蛋白性肽（SP）使雌性产卵更多，降低重新交配的接受度，消耗更多的食物，并在交配时产生更集中的排泄物。据报道，由于SP刺激产卵，它间接增加了食物消耗，但据报道，它在交配雌性体内产生更集中的排泄物的作用与产卵无关。此外，有研究表明SP对食物消耗的影响会在交配后持续数天，而对排泄的影响是否也是如此尚不清楚。SP对雌性配偶有短暂和长期的影响；后者的发生是因为这种肽与雌性精子结合并缓慢释放。在这里，我们使用了与SP或其调节因子突变的雄性交配的雌性果蝇的排泄时间测量，以测试SP对排泄影响的持续时间。我们发现SP对排泄的影响在交配后至少持续1周，这种持续需要SP与精子结合并从精子中释放出来。尽管SP的这些结合/释放需求与交配后增加产蛋量（以及随之而来的增加食物摄入量）的需求相似，但我们发现，排泄表型的长期变化仅部分取决于雌性体内卵子的存在。我们的数据表明，肠道转运的变化是雌性在交配后逐渐释放与精子结合的SP引起的长期交配后反应的一部分，尽管它并不完全依赖于SP引起的其他长期反应。
Drosophila seminal fluid proteins elicit physiological and behavioral changes in the female after mating. For example, the seminal protein sex peptide (SP) causes females to lay more eggs, reduce receptivity to re-mating, consume more food and produce more concentrated excreta upon mating. It has been reported that SP indirectly increases food consumption as a result of its stimulation of egg production, but its role in producing more concentrated excreta in the mated female was reported to be independent of egg production. Additionally, it has been shown that SP's effect on food consumption persists for several days after mating, while it is unknown whether this is true for its effect on excretion. SP can have both transient and long-term effects on mated females; the latter occur because of the peptide's binding to, and slow release from, sperm in the female. Here we used timed measures of excretion by female flies that had mated to males mutant in SP or in its regulators, to test the duration of SP's effect on excretion. We found that SP's effect on excretion persists for at least ~1 week after mating, and that this persistence requires that SP bind to and be released from sperm. Although these binding/release requirements of SP are similar to those for increased egg production (and consequent increased food intake) following mating, we find that the long-term change in excretion phenotype is only partially dependent on the presence of eggs in the female. Our data indicate that a change in intestinal transit is part of the long-term post-mating response elicited by the gradual release of sperm-bound SP in the female after mating, even though it is not fully dependent of other long-term responses elicited by SP.
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DOI：
10.1073/pnas.1631700100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Liu, HF;Kubli, E
通讯作者：
Kubli, E
影响因子：
9.2
作者：
Carvalho, GB;Kapahi, P;Benzer, S
通讯作者：
Benzer, S
果蝇中持续的交战反应需要多种精确的液体蛋白。
影响因子：
4.5
作者：
Ram KR;Wolfner MF
通讯作者：
Wolfner MF
影响因子：
64.5
作者：
BOSWELL, RE;MAHOWALD, AP
通讯作者：
MAHOWALD, AP
影响因子：
29
作者：
Cognigni P;Bailey AP;Miguel-Aliaga I
通讯作者：
Miguel-Aliaga I