MicroRNA-134 as a potential plasma biomarker for the diagnosis of acute pulmonary embolism.
MicroRNA-134 as a potential plasma biomarker for the diagnosis of acute pulmonary embolism.
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MicroRNA-134作为诊断急性肺栓塞的潜在血浆生物标志物
DOI：
10.1186/1479-5876-9-159
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发表时间：
2011-09-24
影响因子：
7.4
通讯作者：
Chen YH
中科院分区：
文献类型：
--
作者：
Xiao J;Jing ZC;Ellinor PT;Liang D;Zhang H;Liu Y;Chen X;Pan L;Lyon R;Liu Y;Peng LY;Liang X;Sun Y;Popescu LM;Condorelli G;Chen YH
背景急性肺栓塞（APE）由于临床表现多变且缺乏可靠的筛查工具，其诊断仍是一个挑战。microRNA（miRNAs）在广泛的病理生理过程中调节基因表达。循环miRNA是心力衰竭，2型糖尿病和其他疾病状态的新兴生物标志物;然而，使用血浆miRNA作为APE诊断的生物标志物仍然是未知的。MethodsThirty-two APE患者，32名健康对照，和22名非APE患者（报告呼吸困难，胸痛，或咳嗽）参加了这项研究。TaqMan miRNA微阵列用于鉴定APE患者血浆中失调的miRNA。采用TaqMan实时定量逆转录聚合酶链反应（RT-PCR）技术对表达异常的miRNA进行验证。结果APE患者血浆中miRNA-134（miR-134）水平显著高于健康对照组和非APE患者，差异有统计学意义（P < 0. 05）。ROC曲线显示血浆miR-134是APE的特异性诊断预测因子，曲线下面积为0.833（95%可信区间为0.737 ~ 0.929; P < 0.001）。由于这一发现，迫切需要进行大规模的研究，为从基础研究到临床应用铺平道路。
BackgroundAcute pulmonary embolism (APE) remains a diagnostic challenge due to a variable clinical presentation and the lack of a reliable screening tool. MicroRNAs (miRNAs) regulate gene expression in a wide range of pathophysiologic processes. Circulating miRNAs are emerging biomarkers in heart failure, type 2 diabetes and other disease states; however, using plasma miRNAs as biomarkers for the diagnosis of APE is still unknown.MethodsThirty-two APE patients, 32 healthy controls, and 22 non-APE patients (reported dyspnea, chest pain, or cough) were enrolled in this study. The TaqMan miRNA microarray was used to identify dysregulated miRNAs in the plasma of APE patients. The TaqMan-based miRNA quantitative real-time reverse transcription polymerase chain reactions were used to validate the dysregulated miRNAs. The receiver-operator characteristic (ROC) curve analysis was conducted to evaluate the diagnostic accuracy of the miRNA identified as the candidate biomarker.ResultsPlasma miRNA-134 (miR-134) level was significantly higher in the APE patients than in the healthy controls or non-APE patients. The ROC curve showed that plasma miR-134 was a specific diagnostic predictor of APE with an area under the curve of 0.833 (95% confidence interval, 0.737 to 0.929; P < 0.001).ConclusionsOur findings indicated that plasma miR-134 could be an important biomarker for the diagnosis of APE. Because of this finding, large-scale investigations are urgently needed to pave the way from basic research to clinical utilization.
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影响因子：
9.3
作者：
Adachi, Taichi;Nakanishi, Michio;Iwai, Naoharu
通讯作者：
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DOI：
10.1038/nrrheum.2010.81
发表时间：
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期刊：
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