Dosage-dependent deregulation of an AGAMOUS-LIKE gene cluster contributes to interspecific incompatibility.
Dosage-dependent deregulation of an AGAMOUS-LIKE gene cluster contributes to interspecific incompatibility.
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DOI：
10.1016/j.cub.2009.05.068
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发表时间：
2009-07-14
期刊：
影响因子：
--
通讯作者：
Comai L
中科院分区：
文献类型：
--
作者：
Walia H;Josefsson C;Dilkes B;Kirkbride R;Harada J;Comai L
种间杂种的合子后致死性可能是由于基因表达、拷贝数或编码序列的差异造成的，并且可以通过改变亲本基因组剂量来克服。在拟南芥和拟南芥之间的杂交中，胚胎停滞与胚乳过度增殖和发育迟缓相关，类似于父本过量间倍体杂交和多梳抑制复合体（PRC）突变体。失败伴随着亲本特异性基因沉默的丧失，包括被 PRC 抑制的三个基因的失调。增加母体基因组剂量可以挽救种子发育和基因沉默。失败种子转录组中上调的基因组编码假定的 AGAMOUS-LIKE MADS 结构域转录因子 (AGL)，该转录因子在正常早期胚乳中表达，并在之前的酵母 2 杂交分析中显示出相互作用。细胞化时这些 AGL 表达的抑制需要 PRC。在种子失败之前，PRC 成员 FIS2 的表达随着 AGL 簇的过度表达而下降。灭活两个成员 AGL62 和 AGL90，减弱了拟南芥和沙拟南芥之间的合子后屏障。我们提出了一个模型，其中剂量敏感的 PRC 功能丧失会导致 AGL 网络失调，这对早期种子发育不利。
Postzygotic lethality of interspecies hybrids can result from differences in gene expression, copy number or coding sequence and can be overcome by altering parental genome dosage. In crosses between Arabidopsis thaliana and A. arenosa, embryo arrest is associated with endosperm hyperproliferation and delayed development similar to paternal-excess interploidy crosses and polycomb repressive complex (PRC) mutants. Failure is accompanied by parent-specific loss of gene silencing including the dysregulation of three genes suppressed by PRC. Increasing the maternal genome dosage rescues seed development and gene silencing. A gene set upregulated in the failing seed transcriptome encoded putative AGAMOUS-LIKE MADS domain transcription factors (AGL) that were expressed in normal early endosperm and were shown to interact in a previous yeast-2 hybrid analysis. Suppression of these AGLs expression upon cellularization required PRC. Preceding seed failure, expression of the PRC member FIS2 decreased concomitant with over-expression of the AGL cluster. Inactivating two members, AGL62 and AGL90, attenuated the postzygotic barrier between A. thaliana and A. arenosa. We present a model where dosage-sensitive loss of PRC function results in a dysregulated AGL network, which is detrimental for early seed development.
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