Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps.
Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps.
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DOI：
10.1590/s1678-77572009000500031
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Cavalcanti BN
中科院分区：
文献类型：
--
作者：
Silva AC;Faria MR;Fontes A;Campos MS;Cavalcanti BN
在侵袭牙髓后，一些细胞产生细胞因子以启动和控制炎症过程。在这些细胞因子中，白细胞介素-1 β（IL-1 β）和白细胞介素-8（IL-8）成为重要的细胞因子。目的：本研究的目的是分析这些细胞因子在健康和炎症牙髓中的位置，分布和浓度。材料和方法：从健康的第三磨牙（n=10）和牙髓切除术（n=10）获得的20个牙髓用于研究，每组一半用于免疫组织化学，一半用于蛋白质提取和ELISA测定。为了模拟对细胞培养物的攻击，还使用了从健康牙髓中获得的成纤维细胞，用大肠杆菌脂多糖（LPS）刺激或不刺激成纤维细胞，并通过ELISA分析IL-1 β和IL-8作为补充信息。对免疫组化获得的数据进行定性分析。从ELISA测定（组织和细胞）获得的数据通过t检验进行统计学处理（p<0.05）。结果如下：免疫组织化学，观察到发炎的牙髓强烈染色的炎症细胞中的两种细胞因子，而健康的牙髓没有免疫标记。来自组织的ELISA定量证实了两种细胞因子的较高存在。此外，培养的牙髓成纤维细胞经LPS刺激后也比对照细胞产生更多的细胞因子。结论：可以得出结论，发炎的牙髓呈现比健康牙髓更高量的IL-1 β和IL-8，并且细菌LPS刺激的牙髓成纤维细胞产生比对照组更高水平的IL-1 β和IL-8。
After aggression to the dental pulp, some cells produce cytokines in order to start and control the inflammatory process. Among these cytokines, interleukin-1 beta (IL-1ß) and interleukin-8 (IL-8) emerge as important ones. Objective: The purpose of this study was to analyze the location, distribution and concentration of these cytokines in healthy and inflamed dental pulps. Material and methods: Twenty pulps, obtained from healthy third molars (n=10) and from pulpectomies (n=10) were used for the study, with half of each group used for immunohistochemistry and half for protein extraction and ELISA assays. Fibroblasts obtained from healthy dental pulps, stimulated or not by Escherichia coli lipopolysaccharide (LPS), in order to simulate aggression on the cell cultures, were also used and analyzed by ELISA for IL-1ß and IL-8 as complementary information. Data obtained from immunohistochemistry were qualitatively analyzed. Data obtained from ELISA assays (tissue and cells) were statistically treated by the t-test (p<0.05). Results: Immunohistochemically, it was observed that inflamed pulps were strongly stained for both cytokines in inflammatory cells, while healthy pulps were not immunolabeled. ELISA from tissues quantitatively confirmed the higher presence of both cytokines. Additionally, cultured pulp fibroblasts stimulated by LPS also produce more cytokines than the control cells. Conclusions: It may be concluded that inflamed pulps present higher amounts of IL-1ß and IL-8 than healthy pulps and that pulp fibroblasts stimulated by bacterial LPS produce higher levels of IL-1ß and IL-8 than the control group.
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影响因子：
3
作者：
DSOUZA, R;BROWN, LR;LACHMAN, LB
通讯作者：
LACHMAN, LB
影响因子：
4.2
作者：
Jiang, YL;Russell, TR;Graves, DT
通讯作者：
Graves, DT
影响因子：
4.2
作者：
Barkhordar, RA;Ghani, QP;Hussain, MZ
通讯作者：
Hussain, MZ
DOI：
10.1016/s1079-2104(99)70118-6
发表时间：
1999-08-01
影响因子：
--
作者：
Huang, GTJ;Potente, AP;Zhang, XL
通讯作者：
Zhang, XL
影响因子：
4.2
作者：
Lertchirakarn, V;Birner, R;Messer, HH
通讯作者：
Messer, HH