Dielectrophoretic separation of microalgae cells in ballast water in a microfluidic chip
Dielectrophoretic separation of microalgae cells in ballast water in a microfluidic chip
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微流控芯片中介电泳分离压载水中的微藻细胞
DOI：
10.1002/elps.201800302
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发表时间：
2018-09
期刊：
影响因子：
2.9
通讯作者：
Wang Wei
中科院分区：
文献类型：
--
作者：
Wang Yanjuan;Wang Junsheng;Wu Xudong;Jiang Zong;Wang Wei
船舶压载水的成分复杂，含有大量的微藻细胞、细菌、微塑料和其他微粒。为了提高压载水中微藻细胞检测的准确性和效率，提出了一种基于交流介电电泳的微流控芯片。在这种微流控芯片中，一片三维电极嵌入在微通道的一侧，八个离散电极布置在微通道的另一侧。为了提高电场分布的不均匀性，增强介电泳力，在电极间设计了绝缘三角形结构。设计鞘流使微粒聚集在电极附近，从而增加所受的DEP力，提高分离效率。为了证明微流控分离芯片的性能，我们开发了两种微藻细胞（扁藻和新月藻）和一种微塑料作为测试样品。对相关参数进行了分析，并利用所设计的微流控芯片进行了分离实验。结果表明，该方法能有效分离混合物中的微藻细胞，首次在微流控芯片上同时利用负、正DEP力分离两种或两种以上的微藻细胞，且具有操作简单、效率高、成本低、体积小等优点，在压载水现场预处理中具有很大的应用潜力。
The composition of the ship's ballast water is complex and contains a large number of microalgae cells, bacteria, microplastics, and other microparticles. To increase the accuracy and efficiency of detection of the microalgae cells in ballast water, a new microfluidic chip for continuous separation of microalgae cells based on alternating current dielectrophoresis was proposed. In this microfluidic chip, one piece of 3‐dimensional electrode is embedded on one side and eight discrete electrodes are arranged on the other side of the microchannel. An insulated triangular structure between electrodes is designed for increasing the inhomogeneity of the electric field distribution and enhancing the dielectrophoresis (DEP) force. A sheath flow is designed to focus the microparticles near the electrode, so as to increase the suffered DEP force and improve separation efficiency. To demonstrate the performance of the microfluidic separation chip, we developed two species of microalgae cells (Platymonas and Closterium) and a kind of microplastics to be used as test samples. Analyses of the related parameters and separation experiments by our designed microfluidic chip were then conducted. The results show that the presented method can separate the microalgae cells from the mixture efficiently, and this is the first time to separate two or more species of microalgae cells in a microfluidic chip by using negative and positive DEP force simultaneously, and moreover it has some advantages including simple operation, high efficiency, low cost, and small size and has great potential in on‐site pretreatment of ballast water.
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DOI：
10.3390/s151229848
发表时间：
2015-12-10
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Perestrelo AR;Águas AC;Rainer A;Forte G
通讯作者：
Forte G
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
R. B. Salah;B. Jaouadi;Amin Bouaziz;Kacem Chaari;C. Blecker;C. Deroanne;H. Attia;S. Besbes
通讯作者：
R. B. Salah;B. Jaouadi;Amin Bouaziz;Kacem Chaari;C. Blecker;C. Deroanne;H. Attia;S. Besbes
影响因子：
1.5
作者：
Hadady, Hanieh;Redelman, Doug;Geiger, Emil J.
通讯作者：
Geiger, Emil J.
集成水下微流体装置中微藻细胞尺寸谱的检测
DOI：
10.1016/j.jembe.2015.08.016
发表时间：
2015-12-01
影响因子：
2
作者：
Wang, Junsheng;Song, Younan;Li, Dongqing
通讯作者：
Li, Dongqing
影响因子：
3
作者：
Naravaneni, R;Jamil, K
通讯作者：
Jamil, K