Identification of heterochronic mutants in Caenorhabditis elegans. Temporal misexpression of a collagen::green fluorescent protein fusion gene.
Identification of heterochronic mutants in Caenorhabditis elegans. Temporal misexpression of a collagen::green fluorescent protein fusion gene.
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秀丽隐杆线虫异时突变体的鉴定。
DOI：
10.1093/genetics/149.3.1335
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Rougvie,AE
中科院分区：
文献类型：
--
作者：
Abrahante,JE;Miller,EA;Rougvie,AE
异慢性基因lin-4、lin-14、lin-28和lin-29决定秀丽隐杆线虫侧皮下缝细胞末端分化的时间。我们设计了一种筛选方法，通过鉴定来自col19启动子（异慢性基因通路的下游靶点）的暂时错误表达的突变体，来鉴定参与这种发育时序机制的其他基因。我们将col19启动子与绿色荧光蛋白基因（gfp）融合，并证明融合基因在野生型动物的皮下表达是成体特异性的，并受异慢性基因途径的暂时调控。我们构建了一株由于lin-4突变而无法表达col19::gfp融合构建体的转基因菌株，该突变阻止了接缝细胞的末端分化。我们已经鉴定并鉴定了在lin-4突变背景下恢复col19::gfp表达的26个突变。大多数突变还恢复了在lin-4突变动物中存在缺陷的接缝细胞末端分化程序的其他方面。12个突变是三个先前确定的基因的等位基因，已知是皮下终末分化的适当时机所必需的。其中包括4个新的等位基因lin-42，这是一种异慢性基因，以前只发现了一个等位基因。两个突变定义了一个新的基因，lin-58。当与lin-4分离后，lin-58突变引起早熟缝细胞末端分化，从而定义了异慢性基因通路的新成员。
The heterochronic genes lin-4, lin-14, lin-28, and lin-29 specify the timing of lateral hypodermal seam cell terminal differentiation in Caenorhabditis elegans. We devised a screen to identify additional genes involved in this developmental timing mechanism based on identification of mutants that exhibit temporal misexpression from the col-19 promoter, a downstream target of the heterochronic gene pathway. We fused the col-19 promoter to the green fluorescent protein gene (gfp) and demonstrated that hypodermal expression of the fusion gene is adult-specific in wild-type animals and temporally regulated by the heterochronic gene pathway. We generated a transgenic strain in which the col-19::gfp fusion construct is not expressed because of mutation of lin-4, which prevents seam cell terminal differentiation. We have identified and characterized 26 mutations that restore col-19::gfp expression in the lin-4 mutant background. Most of the mutations also restore other aspects of the seam cell terminal differentiation program that are defective in lin-4 mutant animals. Twelve mutations are alleles of three previously identified genes known to be required for proper timing of hypodermal terminal differentiation. Among these are four new alleles of lin-42, a heterochronic gene for which a single allele had been described previously. Two mutations define a new gene, lin-58. When separated from lin-4, the lin-58 mutations cause precocious seam cell terminal differentiation and thus define a new member of the heterochronic gene pathway.
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秀丽隐杆线虫皮下细胞分化的替代时间控制系统
影响因子：
64.8
作者：
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