Genetic characterization of the tick-borne orbiviruses.

Genetic characterization of the tick-borne orbiviruses.
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DOI:
10.3390/v7052185
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发表时间:
2015-04-28
期刊:
Viruses
影响因子:
--
通讯作者:
Mertens PP
Mertens PP
中科院分区:
其他
文献类型:
--
作者:
Belaganahalli MN;Maan S;Maan NS;Brownlie J;Tesh R;Attoui H;Mertens PP

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国际病毒分类委员会(ICTV)确认了四种硬虱传播的环状病毒(TBO):Chenuda病毒、Chobar Gorge病毒、Wad Medani病毒和大岛病毒(环状病毒属,呼肠孤病毒科)。核苷酸(NT)和氨基酸(AA)序列的比较为环状病毒的检测和分类提供了基础,但全基因组序列数据仅适用于大岛病毒物种。我们报告了其他三种TBO的代表性基因组序列(病毒分离株:Chenuda病毒(CNUV);乔巴峡谷病毒(CGV)和Wad Medani病毒(WMV))。系统发育比较表明,TBO与昆虫传播的环状病毒(IBOS)是分开的。CNUV、CGV、WMV和GIV与其他环状病毒有低水平的AA/NT同源性,在保守的POL、T2和T13蛋白/基因上,将它们鉴定为四个不同的病毒种。编码细胞附着的TBO基因组片段,外衣壳蛋白1(OC1),大约是昆虫传播的环状病毒相应片段的一半大小,这有助于解释为什么壁虱传播的环状病毒的基因组小~1kb。
The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in ‘conserved’ Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.
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期刊: PloS one
影响因子: 3.7
作者:
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