In vivo acoustic patterning of endothelial cells for tissue vascularization.
In vivo acoustic patterning of endothelial cells for tissue vascularization.
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DOI：
10.1038/s41598-023-43299-0
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发表时间：
2023-09-26
影响因子：
4.6
通讯作者：
Dalecki, Diane
中科院分区：
文献类型：
--
作者：
Comeau, Eric S.;Vander Horst, Melinda A.;Raeman, Carol H.;Child, Sally Z.;Hocking, Denise C.;Dalecki, Diane
需要制造在结构和功能上模拟天然微血管的微血管网络的策略来解决与组织缺血相关的许多临床病症。这项工作的目的是推进一种新的超声技术，直接在体内制造复杂的，功能性的微血管网络。声学图案化利用超声驻波场（USWF）内的力将细胞或微粒按体积组织成限定的几何组件。研制了一种双换能器系统，通过两个超声场的干涉在体内产生特定部位的USWFs。该系统在体内将注射的细胞或微粒快速图案化成胶原水凝胶内的平行片。免疫缺陷小鼠侧翼内注射的内皮细胞的声学图案化在图案化后7天内产生灌注的微血管，而非图案化的细胞不能存活。因此，外部施加的超声场引导注射的内皮细胞在体内自组装成灌注的微血管网络。这些研究通过提供第一个概念验证证明，非侵入性的超声介导的细胞图案化可用于直接在体内制造功能性微血管网络，从而将声学图案化推向体内组织工程。
Strategies to fabricate microvascular networks that structurally and functionally mimic native microvessels are needed to address a host of clinical conditions associated with tissue ischemia. The objective of this work was to advance a novel ultrasound technology to fabricate complex, functional microvascular networks directly in vivo. Acoustic patterning utilizes forces within an ultrasound standing wave field (USWF) to organize cells or microparticles volumetrically into defined geometric assemblies. A dual-transducer system was developed to generate USWFs site-specifically in vivo through interference of two ultrasound fields. The system rapidly patterned injected cells or microparticles into parallel sheets within collagen hydrogels in vivo. Acoustic patterning of injected endothelial cells within flanks of immunodeficient mice gave rise to perfused microvessels within 7 days of patterning, whereas non-patterned cells did not survive. Thus, externally-applied ultrasound fields guided injected endothelial cells to self-assemble into perfused microvascular networks in vivo. These studies advance acoustic patterning towards in vivo tissue engineering by providing the first proof-of-concept demonstration that non-invasive, ultrasound-mediated cell patterning can be used to fabricate functional microvascular networks directly in vivo.
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影响因子：
4.6
作者：
通讯作者：
--
影响因子：
4
作者：
Comeau, Eric S.;Hocking, Denise C.;Dalecki, Diane
通讯作者：
Dalecki, Diane
影响因子：
2.4
作者：
Garvin, Kelley A.;Dalecki, Diane;Hocking, Denise C.
通讯作者：
Hocking, Denise C.
影响因子：
2.9
作者：
Garvin, Kelley A.;Hocking, Denise C.;Dalecki, Diane
通讯作者：
Dalecki, Diane
DOI：
10.1016/j.mtbio.2021.100110
发表时间：
2021-03
期刊：
Materials today. Bio
影响因子：
--
作者：
Guex AG;Di Marzio N;Eglin D;Alini M;Serra T
通讯作者：
Serra T