Light-regulated gene repositioning in Arabidopsis.
Light-regulated gene repositioning in Arabidopsis.
复制标题
DOI：
10.1038/ncomms4027
复制
发表时间：
2014
影响因子：
16.6
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Feng CM;Qiu Y;Van Buskirk EK;Yang EJ;Chen M
植物基因组对环境光信号极其敏感，并且可以通过环境光信号进行发育重编程。在这里，使用滚环扩增的基因特异性的可环化的寡核苷酸加上荧光原位杂交，我们证明，光触发拟南芥光诱导叶绿素a/b结合蛋白（CAB）基因座的快速重新定位从核内部到核周边在其转录激活。CAB重新定位由红色/远红色光感受器光敏色素（PHYs）介导，并被PHYs信号传导的阻遏物（包括COP 1、DET 1和PIFs）抑制。CAB的重新定位似乎是一个单独的调节步骤，发生在其完全转录激活。此外，光诱导位点RBCS，PC和GUN 5在其转录激活过程中经历类似的重新定位行为。我们的研究支持光依赖性基因调控机制，其中PHY通过将它们重新定位到核外围来激活光诱导位点;它还为植物界基因定位的生物学重要性提供了证据。
Plant genomes are extremely sensitive to, and can be developmentally reprogrammed by environmental light cues. Here using rolling-circle amplification of gene-specific circularizable oligonucleotides coupled with fluorescence in situ hybridization, we demonstrate that light triggers a rapid repositioning of the Arabidopsis light-inducible chlorophyll a/b-binding proteins (CAB) locus from the nuclear interior to the nuclear periphery during its transcriptional activation. CAB repositioning is mediated by the red/far-red photoreceptors phytochromes (PHYs) and is inhibited by repressors of PHY signalling, including COP1, DET1 and PIFs. CAB repositioning appears to be a separate regulatory step occurring before its full transcriptional activation. Moreover, the light-inducible loci RBCS, PC and GUN5 undergo similar repositioning behaviour during their transcriptional activation. Our study supports a light-dependent gene regulatory mechanism in which PHYs activate light-inducible loci by relocating them to the nuclear periphery; it also provides evidence for the biological importance of gene positioning in the plant kingdom.
登录
查看更多内容
影响因子：
7.4
作者：
Hamazato, F;Shinomura, T;Furuya, M
通讯作者：
Furuya, M
影响因子：
5.3
作者：
Dieppois, Guennaelle;Iglesias, Nahid;Stutz, Francoise
通讯作者：
Stutz, Francoise
影响因子：
11.8
作者：
Brickner, Donna Garvey;Ahmed, Sara;Brickner, Jason H.
通讯作者：
Brickner, Jason H.
影响因子：
11.6
作者：
Jasencakova, Z;Meister, A;Schubert, I
通讯作者：
Schubert, I
影响因子：
7.4
作者：
KARLINNEUMANN, GA;SUN, L;TOBIN, EM
通讯作者：
TOBIN, EM