The Campylobacter jejuni Response Regulator and Cyclic-Di-GMP Binding CbrR Is a Novel Regulator of Flagellar Motility.
The Campylobacter jejuni Response Regulator and Cyclic-Di-GMP Binding CbrR Is a Novel Regulator of Flagellar Motility.
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DOI：
10.3390/microorganisms10010086
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发表时间：
2021-12-31
期刊：
影响因子：
4.5
通讯作者：
Thompson SA
中科院分区：
文献类型：
--
作者：
Cox CA;Bogacz M;El Abbar FM;Browning DD;Hsueh BY;Waters CM;Lee VT;Thompson SA
空肠弯曲菌是细菌性胃肠炎的主要原因，也与广泛的后遗症有关，包括胃肠外条件，如反应性关节炎和格林-巴利综合征(GBS)。CbrR是一种空肠弯曲菌反应调节因子，被注释为二鸟苷酸环化酶(DGC)，这种酶催化从GTP合成c-di-GMP，这是一种普遍存在的细菌第二信使。在空肠弯曲菌DRH212中，我们构建了一个非标记缺失突变体cbrR−和一个互补突变体cbrR+。运动性分析表明cbrR−表现为高运动性，而cbrR+缺乏运动性。CbrR+中CbrR的过度表达伴随着主要鞭毛蛋白FlaA的表达降低。生物膜分析和扫描电子显微镜显示，DRH212和cbrR−相似，但cbrR+不能形成显著的生物膜。透射电子显微镜显示三个菌株的细胞形态相似，但cbrR+细胞缺乏鞭毛。径向配基差示毛细管作用分析(DRaCALA)显示CbrR与GTP和c-di-GMP结合。在空肠弯曲菌和表达CbrR的大肠杆菌中检测到低水平的c-di-GMP。因此，CbrR是FlaA表达和运动性的负调节因子，而FlaA是空肠弯曲菌致病的关键毒力因子。
A leading cause of bacterial gastroenteritis, Campylobacter jejuni is also associated with broad sequelae, including extragastrointestinal conditions such as reactive arthritis and Guillain-Barré Syndrome (GBS). CbrR is a C. jejuni response regulator that is annotated as a diguanylate cyclase (DGC), an enzyme that catalyzes the synthesis of c-di-GMP, a universal bacterial second messenger, from GTP. In C. jejuni DRH212, we constructed an unmarked deletion mutant, cbrR−, and complemented mutant, cbrR+. Motility assays indicated a hyper-motile phenotype associated with cbrR−, whereas motility was deficient in cbrR+. The overexpression of CbrR in cbrR+ was accompanied by a reduction in expression of FlaA, the major flagellin. Biofilm assays and scanning electron microscopy demonstrated similarities between DRH212 and cbrR−; however, cbrR+ was unable to form significant biofilms. Transmission electron microscopy showed similar cell morphology between the three strains; however, cbrR+ cells lacked flagella. Differential radial capillary action of ligand assays (DRaCALA) showed that CbrR binds GTP and c-di-GMP. Liquid chromatography tandem mass spectrometry detected low levels of c-di-GMP in C. jejuni and in E. coli expressing CbrR. CbrR is therefore a negative regulator of FlaA expression and motility, a critical virulence factor in C. jejuni pathogenesis.
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影响因子：
5.2
作者：
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通讯作者：
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