Isolation, characterization, and differentiation of stem cells for cartilage regeneration.
Isolation, characterization, and differentiation of stem cells for cartilage regeneration.
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DOI：
10.1007/s10439-012-0639-8
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发表时间：
2012-10
影响因子：
3.8
通讯作者：
Darling, Eric M.
中科院分区：
文献类型：
--
作者：
Beane, Olivia S.;Darling, Eric M.
组织工程的目标是为因损伤或疾病而受损的组织创造功能性替代品。在许多情况下，受损的组织无法提供活细胞，因此人们开始研究干细胞作为可能的替代方案。尤其是软骨，可能会受益于干细胞的使用，因为该组织的细胞结构较低，无法有效地自我修复。为了满足这一需求，研究人员正在研究几种多能干细胞来源的软骨形成能力，包括成体和胚胎外间充质干细胞（MSC）、胚胎干细胞（ESC）和诱导多能干细胞（iPSC）。比较研究表明，每种细胞类型都有优点和缺点，虽然很难进行直接比较，但已发表的数据表明，某些来源可能比其他细胞更有希望促进软骨再生。在这篇综述中，我们确定了从骨髓、脂肪、滑膜、肌肉和外周血中分离和软骨分化 MSC 以及来自胚外组织、ESC 和 iPSC 的当前方法。此外，我们还评估生长因子的软骨形成诱导作用，确定用于每种干细胞类型的标准混合物。还包括纯细胞（沉淀）和基于支架的研究，以及体内结果的讨论。
The goal of tissue engineering is to create a functional replacement for tissues damaged by injury or disease. In many cases, impaired tissues cannot provide viable cells, leading to the investigation of stem cells as a possible alternative. Cartilage, in particular, may benefit from the use of stem cells since the tissue has low cellularity and cannot effectively repair itself. To address this need, researchers are investigating the chondrogenic capabilities of several multipotent stem cell sources, including adult and extra-embryonic mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs). Comparative studies indicate that each cell type has advantages and disadvantages, and while direct comparisons are difficult to make, published data suggest some sources may be more promising for cartilage regeneration than others. In this review, we identify current approaches for isolating and chondrogenically differentiating MSCs from bone marrow, fat, synovium, muscle, and peripheral blood, as well as cells from extra-embyronic tissues, ESCs, and iPSCs. Additionally, we assess chondrogenic induction with growth factors, identifying standard cocktails used for each stem cell type. Cell-only (pellet) and scaffold-based studies are also included, as is a discussion of in vivo results.
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DOI：
10.1016/j.biocel.2009.12.011
发表时间：
2010-05
影响因子：
4
作者：
Choi, Young H.;Burdick, Marie D.;Strieter, Robert M.
通讯作者：
Strieter, Robert M.
影响因子：
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作者：
Bailey, Mark M.;Wang, Limin;Detamore, Michael S.
通讯作者：
Detamore, Michael S.
影响因子：
14
作者：
Awad, HA;Wickham, MQ;Guilak, F
通讯作者：
Guilak, F
影响因子：
2.4
作者：
Darling, Eric M.;Topel, Matthew;Guilak, Farshid
通讯作者：
Guilak, Farshid
DOI：
10.1016/j.bbrc.2006.02.171
发表时间：
2006-05-05
影响因子：
3.1
作者：
Chiou, M;Xu, Y;Longaker, MT
通讯作者：
Longaker, MT