Gastric microbial community profiling reveals a dysbiotic cancer-associated microbiota.
Gastric microbial community profiling reveals a dysbiotic cancer-associated microbiota.
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DOI：
10.1136/gutjnl-2017-314205
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发表时间：
2018-03
期刊：
影响因子：
24.5
通讯作者：
Figueiredo C
中科院分区：
文献类型：
--
作者：
Ferreira RM;Pereira-Marques J;Pinto-Ribeiro I;Costa JL;Carneiro F;Machado JC;Figueiredo C
胃癌的发生是由幽门螺杆菌引起的。慢性H.幽门螺杆菌感染导致胃酸分泌减少，这可能允许不同的胃细菌群落生长。微生物组的这种变化可能会增加对胃粘膜的侵袭并导致恶性肿瘤。我们的目的是评估慢性胃炎和胃癌中胃微生物群的组成。采用16S rRNA基因谱分析技术，采用下一代测序技术，对54例胃癌患者和81例慢性胃炎患者的胃微生物群进行了回顾性研究。使用线性判别分析效应量评估两个患者组的微生物组成差异。最相关的分类群和临床诊断之间的关联进行了验证，通过实时定量PCR。用PICRUSt获得微生物群落的预测功能谱。胃癌微生物群的特征是微生物多样性减少，螺杆菌丰度降低和其他细菌属的富集，主要以肠道菌为代表。将这些分类群组合成微生物生态失调指数揭示了生态失调具有区分胃炎和胃癌的优异能力。对微生物群功能特征的分析与癌中亚硝化微生物群落的存在是一致的。在来自不同地理来源的验证队列中证实了主要观察结果。对胃微生物群的详细分析首次揭示，胃癌患者表现出具有遗传毒性潜力的生态失调微生物群落，这与慢性胃炎患者不同。
Gastric carcinoma development is triggered by Helicobacter pylori. Chronic H. pylori infection leads to reduced acid secretion, which may allow the growth of a different gastric bacterial community. This change in the microbiome may increase aggression to the gastric mucosa and contribute to malignancy. Our aim was to evaluate the composition of the gastric microbiota in chronic gastritis and in gastric carcinoma. The gastric microbiota was retrospectively investigated in 54 patients with gastric carcinoma and 81 patients with chronic gastritis by 16S rRNA gene profiling, using next-generation sequencing. Differences in microbial composition of the two patient groups were assessed using linear discriminant analysis effect size. Associations between the most relevant taxa and clinical diagnosis were validated by real-time quantitative PCR. Predictive functional profiling of microbial communities was obtained with PICRUSt. The gastric carcinoma microbiota was characterised by reduced microbial diversity, by decreased abundance of Helicobacter and by the enrichment of other bacterial genera, mostly represented by intestinal commensals. The combination of these taxa into a microbial dysbiosis index revealed that dysbiosis has excellent capacity to discriminate between gastritis and gastric carcinoma. Analysis of the functional features of the microbiota was compatible with the presence of a nitrosating microbial community in carcinoma. The major observations were confirmed in validation cohorts from different geographic origins. Detailed analysis of the gastric microbiota revealed for the first time that patients with gastric carcinoma exhibit a dysbiotic microbial community with genotoxic potential, which is distinct from that of patients with chronic gastritis.
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影响因子：
4.6
作者：
Aviles-Jimenez F;Vazquez-Jimenez F;Medrano-Guzman R;Mantilla A;Torres J
通讯作者：
Torres J
影响因子：
3.7
作者：
Li XX;Wong GL;To KF;Wong VW;Lai LH;Chow DK;Lau JY;Sung JJ;Ding C
通讯作者：
Ding C
槐豆新芽杆菌，一种从苦参根瘤中分离出来的共生细菌
DOI：
10.1099/ijs.0.067017-0
发表时间：
2015-02-01
影响因子：
2.8
作者：
Jiao, Yin Shan;Yan, Hui;Chen, Wen Feng
通讯作者：
Chen, Wen Feng
DOI：
10.1056/nejmra0707500
发表时间：
2008-10-30
期刊：
The New England journal of medicine
影响因子：
--
作者：
Kelly, Ciaran P;LaMont, J Thomas
通讯作者：
LaMont, J Thomas
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars