Identification and Characterization of a Mutant PV-PUR Gene Responsible for the Purple Phenotype of Snap Bean (Phaseolus vulgaris L.).
Identification and Characterization of a Mutant PV-PUR Gene Responsible for the Purple Phenotype of Snap Bean (Phaseolus vulgaris L.).
复制标题
DOI：
10.3390/ijms23031265
复制
发表时间：
2022-01-23
影响因子：
5.6
通讯作者：
Feng G
中科院分区：
文献类型：
--
作者：
Liu C;Yang X;Yan Z;Liu D;Feng G
荚色是油豆角的主要经济性状，其中紫色条纹的荚果更吸引人。以油豆角高代自交系A18- 1为材料，通过人工诱变，获得了一个稳定的紫色突变体。为了揭示油豆角紫色外观的遗传因素和遗传途径，本研究以绿色茎黄荚油豆角品种'A18-1'及其经60 Co-γ射线诱变的紫色突变体'pv-pur'为材料，进行了转录组和代谢组分析。转录组分析结果表明，花青素合成途径中的3个基因在突变体pv-pur中表达差异，其中F3′5′H（Phvul.006G018800）的表达量增加，而F3′H（Phvul.004G021200）和ANS（Phvul.002G152700）的表达量下降。花色素苷代谢组学分析表明，野生型和突变体之间的10种代谢产物的含量有显着差异。转录组学和代谢组学的联合分析表明，一种差异代谢产物飞燕草素与Phvul.006G024700、Phvul.002G152700和Phvul.006G018800的差异表达有关。基于野生型和突变体中6种花青素的含量，我们推测突变体pv-pur的紫色外观是由花青素生物合成途径中二氢黄烷醇（DHK）转化为二氢杨梅酮（DHM）的关键酶F3′5′H（Phvul.006G018800）表达增加引起的。研究结果为进一步研究油豆角花色素苷合成的分子机制奠定了基础，并为油豆角不同花色品种的选育提供了理论框架。
Pod color is a major economic trait of snap beans (Phaseolus vulgaris L.), among which the pod with a purple stripe is more attractive to people. A stable purple mutant with purple stripes on the pods was obtained by artificial mutagenesis with the high generation snap bean inbred line ‘A18-1’. In order to reveal the genetic factors and pathways responsible for the purple appearance in snap bean, we performed transcriptome and metabolome analyses using the green stem and yellow pod cultivar ‘A18-1’ and its purple mutant ‘pv-pur’ via 60Co-γ radiation. Transcriptome analysis showed that three genes in the anthocyanin biosynthetic pathway were differentially expressed, among which the expression level of F3′5′H (Phvul.006G018800) was increased in the mutant ‘pv-pur’, while expression of F3′H (Phvul.004G021200) and ANS (Phvul.002G152700) was downregulated. Anthocyanin-targeted metabonomics analysis showed significant differences in the contents of 10 metabolites between the wild type and mutant plants. Combined analysis of transcriptome and metabolomics showed that one differential metabolite, delphinidin, was related to the differential expression of Phvul.006G024700, Phvul.002G152700, and Phvul.006G018800. Based on the levels of six anthocyanins in wild type and mutant plants, we speculative that the purple appearance of the mutant ‘pv-pur’ is caused by the increased expression of F3′5′H (Phvul.006G018800), the key enzyme in the transformation from dihydroflavanol (DHK) to dihydromyricetone (DHM) in the anthocyanin biosynthetic pathway. The results lay a foundation for further studies on the molecular mechanism of anthocyanin synthesis in snap bean, and provide a framework for breeding different colors of snap bean.
登录
查看更多内容
影响因子：
--
作者：
Gould KS
通讯作者：
Gould KS
DOI：
10.3390/molecules23010025
发表时间：
2017-12-22
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Li Y;Luo X;Wu C;Cao S;Zhou Y;Jie B;Cao Y;Meng H;Wu G
通讯作者：
Wu G
影响因子：
1.6
作者：
Okinaka, Y;Shimada, Y;Kikuchi, Y
通讯作者：
Kikuchi, Y
芸豆荚花色苷组成及花色苷生物合成基因表达分析
影响因子：
6.5
作者：
Hu, Jingtao;Chen, Guoping;Hu, Zongli
通讯作者：
Hu, Zongli
影响因子：
1.6
作者：
Nakamura, Noriko;Fukuchi-Mizutani, Masako;Tanaka, Yoshikazu
通讯作者：
Tanaka, Yoshikazu