Estrogenic environmental contaminants alter the mRNA abundance profiles of genes involved in gonadal differentiation of the American bullfrog.
Estrogenic environmental contaminants alter the mRNA abundance profiles of genes involved in gonadal differentiation of the American bullfrog.
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DOI：
10.1016/j.scitotenv.2015.02.033
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发表时间：
2015-07-15
影响因子：
9.8
通讯作者：
Propper, Catherine R.
中科院分区：
文献类型：
--
作者：
Wolff, Stephanie E.;Veldhoen, Nik;Helbing, Caren C.;Ramirez, Claire A.;Malpas, Janae M.;Propper, Catherine R.
关键词：
野生动物和人类接触到环境中可能对正常生殖功能和发育产生不利影响的人为化学品混合物。我们确定了暴露于雌激素化学品和废水（WWE）对美国牛蛙，蛙（Lithobates）catesbeiana，一个物种，其广泛分布的性腺发育的影响，使其成为一个理想的模型化学污染物的内分泌效应的环境监测。将变性前的牛蛙蝌蚪暴露于处理载体17β-雌二醇（E2; 10−9 M）或4-叔辛基苯酚（OP; 10−9 M、10−8 M和10−7 M）。此外，性腺分化进行了评估牛蛙蝌蚪从WWE包含网站与那些从参考位置接收没有WWE。在这两项研究中，表型性别，类固醇生成因子-1（nr 5a 1），芳香化酶（cyp 19 a1）的mRNA水平，使用定量实时PCR进行了测定。暴露于E2或OP不会改变性别比例。在对照组中，nr 5a 1和cyp 19 a1的转录水平表现出性别二型性，男性表现出较高的nr 5a 1水平和女性更丰富的cyp 19 a1。然而，E2暴露增加cyp 19 a1 mRNA丰度在睾丸和卵巢中的水平下降，消除在对照组中观察到的性二型。与对照组相比，E2暴露的雄性动物睾丸中的nr 5a 1转录水平增加，而雌性动物则无E2效应。OP处理对雌性cyp 19 a1 mRNA丰度没有影响，但暴露于10−7 M OP会增加睾丸转录水平。用10 - 9和10 - 8 M OP处理，而不是10 - 7 M，导致卵巢和睾丸中nr 5a 1转录本的丰度降低。来自该领域的动物cyp 19 a1的性二型性腺水平，但两种性别的WWE网站表现出升高的cyp 19 a1转录丰度相比，参考位置。分散在环境中的单个化学化合物和人为废水影响性腺mRNA编码参与性腺分化的关键蛋白质的水平。
Wildlife and human populations are exposed to anthropogenic mixtures of chemicals in the environment that may adversely influence normal reproductive function and development. We determined the effects of exposure to estrogenic chemicals and wastewater effluent (WWE) on developing gonads of the American bullfrog, Rana (Lithobates) catesbeiana, a species whose widespread distribution make it an ideal model for environmental monitoring for endocrine effects of chemical contaminants. Premetamorphic bullfrog tadpoles were exposed to treatment vehicle, 17β-estradiol (E2; 10−9 M) or 4-tert-octylphenol (OP; 10−9 M, 10−8 M, and 10−7 M). Additionally, gonadal differentiation was evaluated in bullfrog tadpoles from a WWE-containing site versus those from a reference location receiving no WWE. In both studies, phenotypic sex, steroidogenic factor-1 (nr5a1), and aromatase (cyp19a1) mRNA levels using quantitative real-time PCR were determined. Exposure to E2 or OP did not alter sex ratios. In controls, both nr5a1 and cyp19a1 transcript levels exhibited sexual dimorphism, with males demonstrating higher levels of nr5a1 and females greater abundance of cyp19a1. However, E2 exposure increased cyp19a1 mRNA abundance in testes and decreased levels in ovaries, eliminating the sexual dimorphism observed in controls. E2-exposed males exhibited increased nr5a1 transcript levels in the testes compared to controls, while females demonstrated no E2 effect. OP treatment had no effect on female cyp19a1 mRNA abundance, but exposure to 10−7 M OP increased testicular transcript levels. Treatment with 10−9 and 10−8 M OP, but not 10−7 M, resulted in decreased abundance of nr5a1 transcript in both ovaries and testes. Animals from the field had sexually dimorphic gonadal levels of cyp19a1, but both sexes from the WWE site exhibited elevated cyp19a1 transcript abundance compared to the reference location. Individual chemical compounds and anthropogenic wastewater effluent dispersed within the environment influence the levels of gonadal mRNA encoding key proteins involved in gonadal differentiation.
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影响因子：
4.8
作者：
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通讯作者：
Crews, D
影响因子：
10.4
作者：
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DOI：
10.1073/pnas.0909519107
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作者：
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影响因子：
10.4
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