Profiling microbial community structures across six large oilfields in China and the potential role of dominant microorganisms in bioremediation
Profiling microbial community structures across six large oilfields in China and the potential role of dominant microorganisms in bioremediation
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分析中国六大油田的微生物群落结构以及优势微生物在生物修复中的潜在作用
DOI：
10.1007/s00253-015-6748-1
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发表时间：
2015-06
影响因子：
5
通讯作者：
Peng Xiaotong
中科院分区：
文献类型：
--
作者：
Sun Weimin;Li Jiwei;Jiang Lei;Sun Zhilei;Fu Meiyan;Peng Xiaotong
成功的石油污染生物修复是建立在对现场物理化学条件和本地微生物群落以及微生物与地球化学变量之间相互作用的全面了解的基础上的。选取中国6个不同地理气候区主要油田的19个油污染土壤样品和5个未污染对照样品，研究土壤微生物生态系统的空间分布特征。微生物群落分析表明，不同油田含油污染土壤微生物多样性差异显著。典型对应分析（CCA）进一步表明，包括土壤湿度和硫酸盐浓度在内的一系列原位地球化学参数是影响整体微生物群落结构和组成的因素之一。系统发育分析表明，绝大多数序列与aarthrobacter属、Dietzia属、Pseudomonas属、Rhodococcus属和marinobacter属有关，其中许多属含有已知的油降解或油乳化菌。值得注意的是，在一些油污染土壤样品中发现了大量的古细菌属，包括hal碱球菌、钠单胞菌、盐terrigena和钠碱菌，表明这些古细菌可能在一些油污染土壤中起着重要的生态作用。该研究为不同污染土壤微生物群落的多样性提供了直接可靠的参考，并可能影响石油污染的原位生物修复策略。
Successful bioremediation of oil pollution is based on a comprehensive understanding of the in situ physicochemical conditions and indigenous microbial communities as well as the interaction between microorganisms and geochemical variables. Nineteen oil-contaminated soil samples and five uncontaminated controls were taken from six major oilfields across different geoclimatic regions in China to investigate the spatial distribution of the microbial ecosystem. Microbial community analysis revealed remarkable variation in microbial diversity between oil-contaminated soils taken from different oilfields. Canonical correspondence analysis (CCA) further demonstrated that a suite of in situ geochemical parameters, including soil moisture and sulfate concentrations, were among the factors that influenced the overall microbial community structure and composition. Phylogenetic analysis indicated that the vast majority of sequences were related to the generaArthrobacter,Dietzia,Pseudomonas,Rhodococcus, andMarinobacter, many of which contain known oil-degrading or oil-emulsifying species. Remarkably, a number of archaeal genera includingHalalkalicoccus,Natronomonas,Haloterrigena, andNatrinemawere found in relatively high abundance in some of the oil-contaminated soil samples, indicating that theseEuryarchaeotamay play an important ecological role in some oil-contaminated soils. This study offers a direct and reliable reference of the diversity of the microbial community in various oil-contaminated soils and may influence strategies for in situ bioremediation of oil pollution.
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影响因子：
5
作者：
Al-Awadhi, H.;Sulaiman, Rasha H. D.;Radwan, S. S.
通讯作者：
Radwan, S. S.
PrimerProspector：条形码聚合酶链反应引物的从头设计和分类分析。
DOI：
10.1093/bioinformatics/btr087
发表时间：
2011-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Walters WA;Caporaso JG;Lauber CL;Berg-Lyons D;Fierer N;Knight R
通讯作者：
Knight R
影响因子：
4.4
作者：
Weimin Sun;Shuguang Xie;Chunling Luo;A. Cupples
通讯作者：
Weimin Sun;Shuguang Xie;Chunling Luo;A. Cupples
影响因子：
3.7
作者：
dos Santos HF;Cury JC;do Carmo FL;dos Santos AL;Tiedje J;van Elsas JD;Rosado AS;Peixoto RS
通讯作者：
Peixoto RS
影响因子：
2.2
作者：
Harwati, Teresia Umi;Kasai, Yuki;Watanabe, Kazuya
通讯作者：
Watanabe, Kazuya