Evaluation of the Bacterial Diversity in the Human Tongue Coating Based on Genus-Specific Primers for 16S rRNA Sequencing.
Evaluation of the Bacterial Diversity in the Human Tongue Coating Based on Genus-Specific Primers for 16S rRNA Sequencing.
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DOI：
10.1155/2017/8184160
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发表时间：
2017
影响因子：
--
通讯作者：
Lu Z
中科院分区：
文献类型：
--
作者：
Sun B;Zhou D;Tu J;Lu Z
舌苔的特征是中医理论中诊断疾病的重要标志。舌背细菌作为口腔微生物群的栖息地，已被证明是许多口腔疾病的病因。高通量下一代测序（NGS）平台已广泛应用于细菌16S rRNA基因的分析。我们开发了一种基于属特异性多引物扩增和基于连接的微生物群测序的方法。为了验证该方法的有效性，我们对6例不同中医类型肺癌患者的舌苔样本进行了深入分析，通过该平台检测到600多个属的细菌。结果表明，基于连接的平行测序结合酶切和多重扩增可扩大测序reads的有效长度，可应用于微生物群分析。
The characteristics of tongue coating are very important symbols for disease diagnosis in traditional Chinese medicine (TCM) theory. As a habitat of oral microbiota, bacteria on the tongue dorsum have been proved to be the cause of many oral diseases. The high-throughput next-generation sequencing (NGS) platforms have been widely applied in the analysis of bacterial 16S rRNA gene. We developed a methodology based on genus-specific multiprimer amplification and ligation-based sequencing for microbiota analysis. In order to validate the efficiency of the approach, we thoroughly analyzed six tongue coating samples from lung cancer patients with different TCM types, and more than 600 genera of bacteria were detected by this platform. The results showed that ligation-based parallel sequencing combined with enzyme digestion and multiamplification could expand the effective length of sequencing reads and could be applied in the microbiota analysis.
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