Functionalization of Electrospun Nanofibers and Fiber Alignment Enhance Neural Stem Cell Proliferation and Neuronal Differentiation.
Functionalization of Electrospun Nanofibers and Fiber Alignment Enhance Neural Stem Cell Proliferation and Neuronal Differentiation.
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电纺纳米纤维的功能化和纤维取向促进神经干细胞的增殖和神经元分化。
DOI：
10.3389/fbioe.2020.580135
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发表时间：
2020
影响因子：
5.7
通讯作者：
Ferreira FC
中科院分区：
文献类型：
--
作者：
Amores de Sousa MC;Rodrigues CAV;Ferreira IAF;Diogo MM;Linhardt RJ;Cabral JMS;Ferreira FC
神经干细胞(NSCs)具有生成神经系统细胞的潜力，当在纳米纤维支架上培养时，构成了一种很有前途的神经组织工程方法。在这项工作中，评估了将纳米纤维取向与具有生物黏附基序的电纺聚己内酯(ε)纳米纤维功能化相结合对神经干细胞系(CGR8-NS)培养的影响。提出了神经干细胞纤维密度的五级分级标准，选择了纤维密度为70~80%的4.5水平进行神经干细胞的体外培养。取向的纳米纤维引导了NSC的分布，特别是在层粘连蛋白(PCL-LN)和含有RGD的多肽GRGDSP(PCL-RGD)的存在下，促进了更高的细胞伸长，通过偏心率和轴比来量化。原位分化后PCL-LN表达Tuj1的细胞比例相对较高，突起发育时间(41.1±1.0μm)明显长于PCL-RGD(32.0±1.0μm)、原始PCL(25.1±1.2μm)或PCL-RGD随机定向纤维(26.5±1.4μm)，提示LN的存在促进了神经元分化。这项研究表明，经RGD功能化的定向纳米纤维在细胞黏附和增殖方面的表现与PCL-LN纤维一样好。完整的LN蛋白的存在改善了神经元的分化结果，这对于该系统在组织工程应用中的使用可能是重要的。
Neural stem cells (NSCs) have the potential to generate the cells of the nervous system and, when cultured on nanofiber scaffolds, constitute a promising approach for neural tissue engineering. In this work, the impact of combining nanofiber alignment with functionalization of the electrospun poly-ε-caprolactone (PCL) nanofibers with biological adhesion motifs on the culture of an NSC line (CGR8-NS) is evaluated. A five-rank scale for fiber density was introduced, and a 4.5 level, corresponding to 70–80% fiber density, was selected for NSC in vitro culture. Aligned nanofibers directed NSC distribution and, especially in the presence of laminin (PCL-LN) and the RGD-containing peptide GRGDSP (PCL-RGD), promoted higher cell elongation, quantified by the eccentricity and axis ratio. In situ differentiation resulted in relatively higher percentage of cells expressing Tuj1 in PCL-LN, as well as significantly longer neurite development (41.1 ± 1.0 μm) than PCL-RGD (32.0 ± 1.0 μm), pristine PCL (25.1 ± 1.2 μm), or PCL-RGD randomly oriented fibers (26.5 ± 1.4 μm), suggesting that the presence of LN enhances neuronal differentiation. This study demonstrates that aligned nanofibers, functionalized with RGD, perform as well as PCL-LN fibers in terms of cell adhesion and proliferation. The presence of the full LN protein improves neuronal differentiation outcomes, which may be important for the use of this system in tissue engineering applications.
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影响因子：
3.9
作者：
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通讯作者：
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