An optimized protocol for indirect organogenesis from root explants of Agapanthus praecox subsp. orientalis ‘Big Blue’
An optimized protocol for indirect organogenesis from root explants of Agapanthus praecox subsp. orientalis ‘Big Blue’
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百子莲根外植体间接器官发生的优化方案。
DOI：
10.3390/horticulturae8080715
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发表时间：
2022-08
期刊：
影响因子：
3.1
通讯作者：
Xiaohui Shen
中科院分区：
文献类型：
--
作者：
Qianwen Tang;Xiangxin Guo;Yuanshan Zhang;Qingyun Li;Guanqun Chen;Huale Sun;Weiming Wang;Xiaohui Shen
仙人掌具有独特的大蓝紫色花序，具有很高的观赏价值，已成为花卉市场上的新兴品种。由于快速进入市场，组织培养技术或器官发生技术比传统的繁殖方法具有诱人的应用前景。在本研究中，成功地建立了一种基于间接器官发生的高效的早熟禾新亚种鉴定方法。东方人‘大蓝’。比较了根尖和根段两种外植体对诱导培养基愈伤组织诱导率的影响。诱导培养基为Murashige和Skoog‘s(MS)基盐+不同浓度的苦参碱(PIC)、2，4-二氯苯氧乙酸(2，4-D)、噻二唑脲(TDZ)、激动素(KT)和萘乙酸(NAA)。在两种类型的外植体中，根尖的愈伤组织诱导效果优于根段。在所试验的诱导培养基中，MS+2 mg/L PIC+1.5 mg/L KT+0.1 mg/L NAA的愈伤组织诱导率最高，达100.00%，这可能是由于内源激素含量较高，尤其是吲哚乙酸含量较高所致。愈伤组织增殖的最适培养基为MS+1.0 mg/L PIC+1.0 mg/L 6-BA+0.4 mg/L NAA，鲜重增加72.74%。转移到不定芽诱导培养基上25d后，从盛开的愈伤组织表面脱分化出不定芽，90d后发育成有根的植株。试管苗移栽温室，成活率达92.86%。本研究创新性地建立了以根为外植体的早熟禾间接器官发生组织培养体系，为其应用提供了实践参考。
Agapanthus praecox has become a burgeoning variety in the flower market due to its high ornamental value with unique large blue-purple inflorescence. For rapid entering into the market, tissue culture technology or organogenesis has an attractive application over the conventional reproduction approach. In this study, a highly efficient protocol based on indirect organogenesis has been successfully established for A. praecox subsp. orientalis ‘Big Blue’. Two types of explants, root tips versus root segments, were compared for callus induction frequency in response to the induction culture media. The induction media contain Murashige and Skoog’s (MS) Basal Salt supplemented with various concentrations of picloram (PIC), 2,4-Dichlorophenoxyacetic acid (2,4-D), thidiazuron (TDZ), kinetin (KT) and naphthalene acetic acid (NAA). Of the two types of explants, root tips were found to be more effective for callus induction than root segments. Among the induction media tested, the highest callus induction rate (100.00%) was achieved when cultured on MS supplemented with 2.0 mg/L PIC, 1.5 mg/L KT and 0.1 mg/L NAA, which was probably accredited to higher endogenous phytohormone contents, especially of 3-indoleacetic (IAA). The optimal medium for callus proliferation was MS + 1.0 mg/L PIC + 1.0 mg/L 6-BA + 0.4 mg/L NAA, and the fresh weight increased by 72.74%. After being transferred onto the adventitious bud induction medium for 25 days, shoots were dedifferentiated from the surface of the flourishing callus, which then developed to the plantlet with roots in 90 days. The plantlets were transplanted in a greenhouse with a survival rate of 92.86%. This study innovatively established an indirect organogenesis tissue culture system of A. praecox with roots as explants, which provided a practical reference in its application.
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影响因子：
0.4
作者：
Sakae Suzuki;M. Nakano
通讯作者：
Sakae Suzuki;M. Nakano
影响因子：
5.9
作者：
E. Zayed;O. H. A. Elbar
通讯作者：
E. Zayed;O. H. A. Elbar
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
He Yue-qiu
通讯作者：
He Yue-qiu
影响因子：
0.8
作者：
J. S. Yaacob;A. Saleh;H. Elias;S. Abdullah;Noraini Mahmad;Normadiha Mohamed
通讯作者：
J. S. Yaacob;A. Saleh;H. Elias;S. Abdullah;Noraini Mahmad;Normadiha Mohamed
影响因子：
1.6
作者：
K. Supaibulwatana;M. Mii
通讯作者：
K. Supaibulwatana;M. Mii