Investigation of Streptococcus salivarius-mediated inhibition of pneumococcal adherence to pharyngeal epithelial cells.
Investigation of Streptococcus salivarius-mediated inhibition of pneumococcal adherence to pharyngeal epithelial cells.
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DOI：
10.1186/s12866-016-0843-z
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发表时间：
2016-09-29
期刊：
影响因子：
4.2
通讯作者：
Satzke C
中科院分区：
文献类型：
--
作者：
Manning J;Dunne EM;Wescombe PA;Hale JD;Mulholland EK;Tagg JR;Robins-Browne RM;Satzke C
肺炎球菌粘附于鼻咽上皮是定植和疾病的关键步骤。益生菌唾液链球菌在体外可以抑制肺炎球菌对上皮细胞的粘附。我们利用人咽部上皮细胞系（Detroit 562）研究了预给药两种不同的唾液链球菌菌株后的抑制机制。虽然唾液链球菌菌株K12和M18的细菌素编码巨质粒对于阻止肺炎球菌在固体培养基上生长是必不可少的，但它们并不需要抑制肺炎球菌的粘附。对唾液链球菌K12和两种肺炎球菌分离株（血清型19F和6A）的实验表明，对19F的抑制可能涉及唾液链球菌介导的肺炎球菌结合位点的阻断：观察到K12与19F的粘附之间呈负相关，当阻止K12接触上皮细胞时不发生抑制。K12介导的6A对粘附的抑制可能涉及其他机制，因为没有观察到K12与6A的粘附之间的相关性，K12可以在没有细胞接触的情况下抑制6A的粘附。这些结果表明，唾液链球菌采用几种机制，包括阻断肺炎球菌结合位点，以减少肺炎球菌对咽上皮细胞的粘附。这些发现扩展了我们对益生菌如何抑制肺炎球菌粘附性的理解，并有助于未来预防肺炎球菌定植的新策略的发展。本文的在线版本（doi:10.1186/s12866-016-0843-z）包含补充材料，可供授权用户使用。
Pneumococcal adherence to the nasopharyngeal epithelium is a critical step in colonisation and disease. The probiotic bacterium, Streptococcus salivarius, can inhibit pneumococcal adherence to epithelial cells in vitro. We investigated the mechanism(s) of inhibition using a human pharyngeal epithelial cell line (Detroit 562) following pre-administration of two different strains of S. salivarius. Whilst the bacteriocin-encoding megaplasmids of S. salivarius strains K12 and M18 were essential to prevent pneumococcal growth on solid media, they were not required to inhibit pneumococcal adherence. Experiments testing S. salivarius K12 and two pneumococcal isolates (serotypes 19F and 6A) showed that inhibition of 19F may involve S. salivarius-mediated blocking of pneumococcal binding sites: a negative correlation was observed between adherence of K12 and 19F, and no inhibition occurred when K12 was prevented from contacting epithelial cells. K12-mediated inhibition of adherence by 6A may involve additional mechanisms, since no correlation was observed between adherence of K12 and 6A, and K12 could inhibit 6A adherence in the absence of cell contact. These results suggest that S. salivarius employs several mechanisms, including blocking pneumococcal binding sites, to reduce pneumococcal adherence to pharyngeal epithelial cells. These findings extend our understanding of how probiotics may inhibit pneumococcal adherence and could assist with the development of novel strategies to prevent pneumococcal colonisation in the future. The online version of this article (doi:10.1186/s12866-016-0843-z) contains supplementary material, which is available to authorized users.
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