Serine/threonine Kinases Play Important Roles in Regulating Polyunsaturated Fatty Acid Biosynthesis in Synechocystis sp. PCC6803.
Serine/threonine Kinases Play Important Roles in Regulating Polyunsaturated Fatty Acid Biosynthesis in Synechocystis sp. PCC6803.
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丝氨酸/苏氨酸激酶在调节合成细胞藻中的多不饱和脂肪酸生物合成中起着重要作用。 PCC6803。
DOI：
10.3389/fbioe.2021.618969
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发表时间：
2021
影响因子：
5.7
通讯作者：
Dai M
中科院分区：
文献类型：
--
作者：
Chen G;Cao Y;Zhong H;Wang X;Li Y;Cui X;Lu X;Bi X;Dai M
丝氨酸/苏氨酸激酶（STKs）在原核细胞的生长、分化和次级代谢等功能中起着重要作用。当外部环境发生变化时，原核生物依赖于信号转导系统，包括快速感知这些变化并改变基因表达以诱导适当代谢变化的STK。在这项研究中，我们研究的作用，STK基因spkD和spkG脂肪酸的生物合成中的单细胞蓝藻集胞藻属。PCC 6803，采用靶向基因敲除。在培养温度为30°C、光照强度为40 μmol·cm-2·s-1的条件下，spkD和spkG基因敲除突变体的亚油酸（C18：2）、γ-亚麻酸（C18：3 n6）、α-亚麻酸（C18：3 n3）和十八碳四烯酸（C18：4）水平显著低于野生型。脂肪酸去饱和酶和STK基因的表达水平在spkD和spkG基因敲除突变体之间存在差异。这些结果表明，spkD和spkG可能直接或间接影响集胞藻6803脂肪酸组成的脂肪酸脱氢酶基因的表达。因此，STK基因spkD和spkG在集胞藻PCC 6803的多不饱和脂肪酸生物合成中起重要作用。这些发现将有助于开发高脂肪酸含量的蓝藻种质资源。为蓝藻的基因工程改造提供了理论依据，提高了蓝藻的次生代谢产物产量，增加了经济效益。
Serine/threonine kinases (STKs) play important roles in prokaryotic cellular functions such as growth, differentiation, and secondary metabolism. When the external environment changes, prokaryotes rely on signal transduction systems, including STKs that quickly sense these changes and alter gene expression to induce the appropriate metabolic changes. In this study, we examined the roles of the STK genes spkD and spkG in fatty acid biosynthesis in the unicellular cyanobacterium Synechocystis sp. PCC6803, using targeted gene knockout. The linoleic acid (C18: 2), γ-linolenic acid (C18: 3n6), α-linolenic acid (C18: 3n3), and stearidonic acid (C18: 4) levels were significantly lower in spkD and spkG gene knockout mutants than in the wild type at a culture temperature of 30°C and a light intensity of 40 μmol⋅m–2⋅s–1. The expression levels of fatty acid desaturases and STK genes differed between the spkD and spkG gene knockout mutants. These observations suggest that spkD and spkG may directly or indirectly affect the fatty acid composition in Synechocystis sp. PCC6803 by regulating the expression of fatty acid desaturases genes. Therefore, the STK genes spkD and spkG play important roles in polyunsaturated fatty acid biosynthesis in Synechocystis sp. PCC6803. These findings could facilitate the development of cyanobacteria germplasm resources that yield high levels of fatty acids. In addition, they provide a theoretical basis for the genetic engineering of cyanobacteria with improved yields of secondary metabolites and increased economic benefits.
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