High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream.
High Representation of Archaea Across All Depths in Oxic and Low-pH Sediment Layers Underlying an Acidic Stream.
复制标题
DOI：
10.3389/fmicb.2020.576520
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Golyshina OV
中科院分区：
文献类型：
--
作者：
Distaso MA;Bargiela R;Brailsford FL;Williams GB;Wright S;Lunev EA;Toshchakov SV;Yakimov MM;Jones DL;Golyshin PN;Golyshina OV
Parys Mountain或Mynydd Parys(英国安格尔西岛)是一个受地雷影响的环境，其中容纳了各种嗜酸微生物。我们之前对其中一条地表酸性溪流的水和沉积物的研究表明，古生菌在整个微生物群落中所占的比例很高。为了了解古菌的空间分布，我们采集了0-20 cm的沉积物岩芯，并对不同岩心层的微生物群进行了化学分析和分类鉴定。测序结果的分类关联表明，古生菌占所有沉积物深度微生物群落的6.2%至54%。大多数古菌与热浆菌目相关，其中最丰富的一组序列与B_DKE系统型聚在一起，其次是“E-等离子”、“A-等离子”，以及其他尚未培养的热浆亚目与铁质和钩体。以较小的比例表现。在所有深度和整个化学条件范围内都发现了热质浆目动物，其丰度与沉积物中Fe、As、Cr和Mn的含量有关。细菌微生物组分主要由变形杆菌门、放线杆菌门、硝化螺门、非培养的氯杆菌(AD3组)和酸杆菌门的成员组成。这项研究揭示了受酸性矿山废水影响的沉积层中热浆菌类的高度丰度，并指出这些生物是碳的主要贡献者，可能也是该生态系统中铁和硫循环的主要贡献者。
Parys Mountain or Mynydd Parys (Isle of Anglesey, United Kingdom) is a mine-impacted environment, which accommodates a variety of acidophilic organisms. Our previous research of water and sediments from one of the surface acidic streams showed a high proportion of archaea in the total microbial community. To understand the spatial distribution of archaea, we sampled cores (0–20 cm) of sediment and conducted chemical analyses and taxonomic profiling of microbiomes using 16S rRNA gene amplicon sequencing in different core layers. The taxonomic affiliation of sequencing reads indicated that archaea represented between 6.2 and 54% of the microbial community at all sediment depths. Majority of archaea were associated with the order Thermoplasmatales, with the most abundant group of sequences being clustered closely with the phylotype B_DKE, followed by “E-plasma,” “A-plasma,” other yet uncultured Thermoplasmatales with Ferroplasma and Cuniculiplasma spp. represented in minor proportions. Thermoplasmatales were found at all depths and in the whole range of chemical conditions with their abundance correlating with sediment Fe, As, Cr, and Mn contents. The bacterial microbiome component was largely composed in all layers of sediment by members of the phyla Proteobacteria, Actinobacteria, Nitrospirae, Firmicutes, uncultured Chloroflexi (AD3 group), and Acidobacteria. This study has revealed a high abundance of Thermoplasmatales in acid mine drainage-affected sediment layers and pointed at these organisms being the main contributors to carbon, and probably to iron and sulfur cycles in this ecosystem.
登录
查看更多内容
影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
DOI：
10.1099/ijsem.0.000725
发表时间：
2016-01-01
影响因子：
2.8
作者：
Golyshina, Olga V.;Luensdorf, Heinrich;Golyshin, Peter N.
通讯作者：
Golyshin, Peter N.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
5.2
作者：
Hamilton TL;Jones DS;Schaperdoth I;Macalady JL
通讯作者：
Macalady JL
DOI：
10.1099/ijs.0.009639-0
发表时间：
2009-11-01
影响因子：
2.8
作者：
Golyshina, Olga V.;Yakimov, Michail M.;Golyshin, Peter N.
通讯作者：
Golyshin, Peter N.