Identification of MicroRNAs Related to Phytohormone Signal Transduction and Self-incompatibility of Rabbiteye Blueberry Pollen
Identification of MicroRNAs Related to Phytohormone Signal Transduction and Self-incompatibility of Rabbiteye Blueberry Pollen
复制标题
兔眼蓝莓花粉植物激素信号转导及自交不亲和性相关MicroRNA的鉴定
DOI：
10.21273/jashs05143-21
复制
发表时间：
2022-11
影响因子：
1.9
通讯作者：
Jie Deng
中科院分区：
文献类型：
--
作者：
Qin Yang;Yan Fu;Tingting Zhang;Shu Peng;Jie Deng
与植物激素信号转导和自交不亲和性相关的microRNAs（miRNAs）可能在花粉直感效应中发挥重要作用。然而，在这方面的相关研究仍然缺乏兔眼蓝莓（越桔ashei）。在本研究中，我们鉴定了miRNAs，预测了它们的靶基因，对靶基因进行了功能富集分析，并筛选了与植物激素信号传导和自交不亲和性相关的miRNAs。共鉴定出491个miRNAs，其中27和67个已知miRNAs以及274和416个新miRNAs分别在兔眼蓝莓品种Brightwell和Premier中发现。与“Premier”相比，“Brightwell”中有31个miRNAs表达上调，62个miRNAs表达下调。京都基因和基因组百科全书（KEGG）功能富集分析表明，预测的4985个靶基因涉及氨基酸生物合成、植物-病原体相互作用和剪接体途径。在兔眼蓝莓花粉中共鉴定出10个、1个、1个、5个、2个、5个和2个分别与生长素、细胞分裂素、赤霉素、脱落酸、乙烯、油菜素类固醇和水杨酸信号传导相关的候选miRNAs。进一步分析表明，novel_miR_49是一个与自交不亲和相关的候选miRNA，其靶基因为maker-VaccDscaff 21-snap-gene-21.37。此外，对novel_miR_49的靶基因的KEGG富集分析表明，它们参与核糖体、氨酰-tRNA生物合成和糖基磷脂酰肌醇-锚生物合成途径。结果表明，兔眼蓝莓花粉microRNA主要通过与生长素、细胞分裂素、赤霉素、脱落酸、乙烯、油菜素内酯、水杨酸等相关的信号转导途径，调控植物激素信号转导和自交不亲和性信号转导。这些结果提示，进一步研究miRNAs在激素信号转导和自交不亲和性调控中的作用，对于阐明花粉直感效应的分子机制是必要的。
MicroRNAs (miRNAs) related to phytohormone signal transduction and self-incompatibility may play an important role in the xenia effect. However, associated research in this area is still lacking in rabbiteye blueberry ( Vaccinium ashei ). In this study, we identified miRNAs, predicted their target genes, performed functional enrichment analysis of the target genes, and screened for miRNAs related to phytohormone signaling and self-incompatibility. A total of 491 miRNAs were identified, of which 27 and 67 known miRNAs as well as 274 and 416 new miRNAs were found in the rabbiteye blueberry cultivars Brightwell and Premier, respectively. Compared with ‘Premier’, 31 miRNAs were upregulated and 62 miRNAs were downregulated in ‘Brightwell’. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis indicated that the 4985 target genes predicted were involved in biosynthesis of amino acids, plant–pathogen interaction, and spliceosome pathways. A total of 10, one, one, five, two, five, and two candidate miRNAs related to auxin, cytokinin, gibberellin, abscisic acid, ethylene, brassinosteroid, and salicylic acid signaling, respectively, in rabbiteye blueberry pollen were identified. Further analysis indicated that novel_miR_49 was a candidate miRNA related to self-incompatibility, and their target gene was maker-VaccDscaff21-snap-gene-21.37 . In addition, the KEGG enrichment analysis of the target genes of novel_miR_49 showed that they were involved in the ribosome, aminoacyl-tRNA biosynthesis, and glycosylphosphatidylinositol-anchor biosynthesis pathways. The results revealed that the microRNAs of rabbiteye blueberry pollen regulated to phytohormone signal transduction and self-incompatibility signal transduction based on related to auxin, cytokinin, gibberellin, abscisic acid, ethylene, brassinosteroid, and salicylic acid signaling. Results suggest that more research of the effects of miRNAs on regulation of hormone signal transduction and self-incompatibility is necessary for elucidating the molecular mechanism of the xenia effect.
登录
查看更多内容
影响因子：
--
作者：
Y. Qi
通讯作者：
Y. Qi
梨果实发育和品质相关miRNA的鉴定
影响因子：
4.4
作者：
Wu J;Wang D;Liu Y;Wang L;Qiao X;Zhang S
通讯作者：
Zhang S
影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
影响因子：
8.7
作者：
Chen C;Zeng Z;Liu Z;Xia R
通讯作者：
Xia R
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO