Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis.
Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis.
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DOI：
10.1016/j.vetmic.2003.07.010
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发表时间：
2004-03-26
影响因子：
3.3
通讯作者：
Möstl K
中科院分区：
文献类型：
--
作者：
Benetka V;Kübber-Heiss A;Kolodziejek J;Nowotny N;Hofmann-Parisot M;Möstl K
猫冠状病毒(FCoV)的毒力差异很大，导致从亚临床病程到致死性猫科感染性腹膜炎(FIP)的一系列临床表现。与毒力变异无关，它们被分为两种不同的类型，I型，原始的FCoV，和II型，它与犬冠状病毒(CCV)密切相关。最近调查了没有FIP的奥地利猫种群中口蹄疫病毒类型的流行情况，表明I型感染占主导地位。然而，FCoV类型在死于FIP的猫身上的分布相当未知。已经开发出了扩增S蛋白基因部分的聚合酶链式反应。设计了类型特异的引物对，产生了不同大小的PCR产物。总共检测了94只组织病理学证实为FIP的猫的器官池。74只猫有明显的分化，其中I型阳性占86%，II型阳性占7%，两型均阳性占7%。这些发现表明，在FIP病例中，FCoV I型也占主导地位，但在14%的病例中检测到FCoV II型，这表明它与FIP病例有关。
Feline coronaviruses (FCoV) vary widely in virulence causing a spectrum of clinical manifestations reaching from subclinical course to fatal feline infectious peritonitis (FIP). Independent of virulence variations they are separated into two different types, type I, the original FCoV, and type II, which is closely related to canine coronavirus (CCV). The prevalence of FCoV types in Austrian cat populations without FIP has been surveyed recently indicating that type I infections predominate. The distribution of FCoV types in cats, which had succumbed to FIP, however, was fairly unknown. PCR assays have been developed amplifying parts of the spike protein gene. Type-specific primer pairs were designed, generating PCR products of different sizes. A total of 94 organ pools of cats with histopathologically verified FIP was tested. A clear differentiation was achieved in 74 cats, 86% of them were type I positive, 7% type II positive, and 7% were positive for both types. These findings demonstrate that in FIP cases FCoV type I predominates, too, nonetheless, in 14% of the cases FCoV type II was detected, suggesting its causative involvement in cases of FIP.
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DOI：
10.1016/0147-9571(89)90062-3
发表时间：
1989-01-01
影响因子：
2
作者：
MOCHIZUKI, M;FURUKAWA, H
通讯作者：
FURUKAWA, H
影响因子：
1.2
作者：
HOHDATSU, T;OKADA, S;KOYAMA, H
通讯作者：
KOYAMA, H
影响因子：
9.4
作者：
HERREWEGH, AAPM;DEGROOT, RJ;ROTTIER, PJM
通讯作者：
ROTTIER, PJM
影响因子：
3.3
作者：
Hohdatsu T;Sasamoto T;Okada S;Koyama H
通讯作者：
Koyama H
影响因子：
2.2
作者：
ADDIE, DD;JARRETT, O
通讯作者：
JARRETT, O