Genomic sequence and phylogenetic analysis of Culex flavivirus, an insect-specific flavivirus, isolated from Culex pipiens (Diptera: Culicidae) in Iowa.
Genomic sequence and phylogenetic analysis of Culex flavivirus, an insect-specific flavivirus, isolated from Culex pipiens (Diptera: Culicidae) in Iowa.
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DOI：
10.1603/033.046.0428
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发表时间：
2009-07
影响因子：
2.1
通讯作者：
Bartholomay LC
中科院分区：
文献类型：
--
作者：
Blitvich BJ;Lin M;Dorman KS;Soto V;Hovav E;Tucker BJ;Staley M;Platt KB;Bartholomay LC
成蚊（双翅目：作为爱荷华州正在进行的虫媒病毒监测工作的一部分，在2007年收集了蚊科），并对包括西尼罗河病毒在内的特定病毒进行了检测。使用黄病毒通用引物通过逆转录聚合酶链反应（RT-PCR）对这些蚊子的一个子集（340个池中的6，061个个体）进行了进一步测试。在211个库蚊库中，对211个库蚊库进行了调查。经检测，50人呈阳性。在51个库中检出1个阳性。Cx的黄病毒最低感染率（表示为每1，000只测试蚊子中阳性蚊子池的数量）。pipiens和Cx.睑板分别为10.3和1.2。在三列伊蚊（Say）（52个样本池）、埃拉特库蚊（Dyar & Knab）（25个样本池）或领土库蚊步行者（1个样本池）中未检测到黄病毒RNA。对所有RT-PCR产物的序列分析显示，这些蚊子感染了库蚊黄病毒（CxFV），这是一种昆虫特异性病毒，以前在日本、印度尼西亚、得克萨斯州、墨西哥、危地马拉和特立尼达分离到。一个分离株的完整基因组进行测序，作为其他八个分离株的包膜蛋白基因。系统发育分析显示，来自美国（爱荷华州和德克萨斯州）的CxFV分离株与来自亚洲的CxFV分离株的关系比来自墨西哥、危地马拉和特立尼达的CxFV分离株的关系更密切。
Adult mosquitoes (Diptera: Culicidae) were collected in 2007 and tested for specific viruses, including West Nile virus, as part of the ongoing arbovirus surveillance efforts in the state of Iowa. A subset of these mosquitoes (6,061 individuals in 340 pools) was further tested by reverse transcription-polymerase chain reaction (RT-PCR) using flavivirus universal primers. Of the 211 pools of Culex pipiens (L.) tested, 50 were positive. One of 51 pools of Culex tarsalis Coquillet was also positive. The flavivirus minimum infection rates (expressed as the number of positive mosquito pools per 1,000 mosquitoes tested) for Cx. pipiens and Cx. tarsalis were 10.3 and 1.2, respectively. Flavivirus RNA was not detected in Aedes triseriatus (Say) (52 pools), Culex erraticus (Dyar & Knab) (25 pools), or Culex territans Walker (one pool). Sequence analysis of all RT-PCR products revealed that the mosquitoes had been infected with Culex flavivirus (CxFV), an insect-specific virus previously isolated in Japan, Indonesia, Texas, Mexico, Guatemala and Trinidad. The complete genome of one isolate was sequenced, as were the envelope protein genes of eight other isolates. Phylogenetic analysis revealed that CxFV isolates from the United States (Iowa and Texas) are more closely related to CxFV isolates from Asia than those from Mexico, Guatemala, and Trinidad.
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影响因子：
3.7
作者：
Kim, Dal Y.;Guzman, Hilda;Tesh, Robert B.
通讯作者：
Tesh, Robert B.
DOI：
10.4269/ajtmh.2009.80.85
发表时间：
2009-01-01
影响因子：
3.3
作者：
Farfan-Ale, Jose A.;Lorono-Pino, Maria A.;Blitvich, Bradley J.
通讯作者：
Blitvich, Bradley J.
影响因子：
2.7
作者：
Crabtree, MB;Sang, RC;Miller, BR
通讯作者：
Miller, BR
影响因子：
3.7
作者：
STOLLAR, V;THOMAS, VL
通讯作者：
THOMAS, VL
影响因子：
3.1
作者：
Kihara, Yuki;Satho, Tomomitsu;Mizutani, Tetsuya
通讯作者：
Mizutani, Tetsuya