Comparative Microbiome Analysis of Three Species of Laboratory-Reared Periplaneta Cockroaches.
Comparative Microbiome Analysis of Three Species of Laboratory-Reared Periplaneta Cockroaches.
复制标题
DOI：
10.3347/kjp.2020.58.5.537
复制
发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Yong TS
中科院分区：
文献类型：
--
作者：
Lee S;Kim JY;Yi MH;Lee IY;Lee WJ;Moon HS;Yong D;Yong TS
蟑螂栖息在各种栖息地，这将影响它们的微生物组。虽然微生物组可能受到饮食和环境因素的影响，但也可能因物种而异。因此，我们进行了16S rDNA靶向高通量测序，以评估3种蟑螂（美洲大蠊、日本大蠊和黑胸大蠊）的微生物组的总体细菌组成，这些蟑螂在相同条件下在实验室中饲养了几代。这些实验是用雄性成年蟑螂进行的。3个种的操作分类单位（OTUs）的数量没有显著差异。就Shannon和Pielou指数而言，美洲黑杨的微生物组值高于日本黑杨和黑杨。还评估了微生物组组成，其中内共生体占日本对虾和黑胸对虾中所有OTU的一半以上。β多样性分析进一步表明，日本对虾和黑胸对虾具有相似的微生物组组成，这与美洲对虾不同。然而，我们还鉴定了日本对虾和黑胸对虾宿主不同的OTU。因此，尽管微生物组组成可能会根据多种条件而变化，但即使在相同条件下饲养个体，也可以在不同的大蠊物种中鉴定出不同的微生物组组成。
Cockroaches inhabit various habitats, which will influence their microbiome. Although the microbiome can be influenced by the diet and environmental factors, it can also differ between species. Therefore, we conducted 16S rDNA-targeted high-throughput sequencing to evaluate the overall bacterial composition of the microbiomes of 3 cockroach species, Periplaneta americana, P. japonica, and P. fuliginosa, raised in laboratory for several generations under the same conditions. The experiments were conducted using male adult cockroaches. The number of operational taxonomic units (OTUs) was not significantly different among the 3 species. With regard to the Shannon and Pielou indexes, higher microbiome values were noted in P. americana than in P. japonica and P. fuliginosa. Microbiome composition was also evaluated, with endosymbionts accounting for over half of all OTUs in P. japonica and P. fuliginosa. Beta diversity analysis further showed that P. japonica and P. fuliginosa had similar microbiome composition, which differed from that of P. americana. However, we also identified that P. japonica and P. fuliginosa host distinct OTUs. Thus, although microbiome compositions may vary based on multiple conditions, it is possible to identify distinct microbiome compositions among different Periplaneta cockroach species, even when the individuals are reared under the same conditions.
登录
查看更多内容
影响因子：
2.9
作者：
Jayasudha, Rajagopalaboopathi;Chakravarthy, Sama Kalyana;Shivaji, Sisinthy
通讯作者：
Shivaji, Sisinthy
DOI：
10.1073/pnas.0907504106
发表时间：
2009-11-17
影响因子：
11.1
作者：
Sabree, Zakee L.;Kambhampati, Srinivas;Moran, Nancy A.
通讯作者：
Moran, Nancy A.
影响因子：
--
作者：
Sabree ZL;Moran NA
通讯作者：
Moran NA
影响因子：
4.4
作者：
Mikaelyan, Aram;Thompson, Claire L.;Brune, Andreas
通讯作者：
Brune, Andreas
影响因子：
2.2
作者：
Vicente CS;Ozawa S;Hasegawa K
通讯作者：
Hasegawa K