Comparative molecular analysis of the prokaryotic diversity of two salt mine soils in southwest China
Comparative molecular analysis of the prokaryotic diversity of two salt mine soils in southwest China
复制标题
西南地区两种盐矿土壤原核生物多样性的比较分子分析
DOI：
10.1002/jobm.201200200
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发表时间：
2013-11
影响因子：
3.1
通讯作者：
崔晓龙
中科院分区：
文献类型：
--
作者：
崔晓龙
虽然人们对高盐环境中的微生物多样性了解甚多，但对盐矿隧道土壤的微生物多样性了解甚少。利用16S rRNA基因克隆文库，对不同盐度和盐组分的一平郎盐矿（YPL）和桥厚盐矿（QH）隧道中存在的古细菌和细菌群落进行系统发育比较。通过扩增的核糖体DNA限制性内切分析，分析了QH的228个序列和YPL的182个序列。文库共发现44个细菌和57个古细菌不同的操作分类单位，分别属于至少8个细菌和3个古细菌分部，但在两个盐矿中并没有观察到所有的分部。QH中属于拟杆菌门（Bacteroidetes）的细菌群落最多（60%），而YPL中属于δ‐Proteobacteria的细菌群落最多（45%）。QH的所有古细菌克隆均隶属于盐杆菌科。相比之下，在YPL文库中，49%的克隆属于盐杆菌科，31%的克隆属于未分类的古菌，21%的克隆属于绿原菌。生物信息学分析和比较表明，两个盐矿间的克隆文库存在显著差异。
While much is known about the microbial diversity in some hypersaline environments, little is known about those of salt mine tunnel soils. The objective of this study was to conduct a comprehensive phylogenetic comparison of the archaeal and bacterial communities present in Yipinglang salt mine (YPL) and Qiaohou salt mine (QH) tunnels differing in salinity and salt composition using 16S rRNA gene clone libraries. Two hundred twenty‐eight sequences for QH and 182 sequences for YPL were analyzed by amplified ribosomal DNA‐restriction analysis. Libraries revealed 44 bacterial and 57 archaeal different operational taxonomic units belonging to at least 8 bacterial and 3 archaeal divisions, but not all divisions were observed in both salt mines. The bacterial community affiliated with the Bacteroidetes was the most abundant (60% of clones) in QH, while the community in YPL was dominated by δ‐Proteobacteria (45% of clones). All archaeal clones from QH were affiliated with Halobacteriaceae. In contrast, in the YPL library, 49% of clones belonged to Halobacteriaceae, 31% of clones related to unclassified archaea, and 21% of clones belonged to Crenarchaeota. Bioinformatic analysis and comparisons showed that the clone libraries were significantly different between two salt mines.
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影响因子：
5.1
作者：
D. Walsh;R. Papke;W. Doolittle
通讯作者：
D. Walsh;R. Papke;W. Doolittle
影响因子：
5.1
作者：
M. Perner;W. Bach;Michael Hentscher;A. Koschinsky;D. Garbe‐Schönberg;W. Streit;H. Strauss
通讯作者：
M. Perner;W. Bach;Michael Hentscher;A. Koschinsky;D. Garbe‐Schönberg;W. Streit;H. Strauss
影响因子：
4.1
作者：
Xiang, Wenliang;Guo, Jianhua;Sun, Qun
通讯作者：
Sun, Qun
影响因子：
2.8
作者：
Stan-Lotter, H;McGenity, TJ;Wanner, G
通讯作者：
Wanner, G
影响因子：
3.7
作者：
Jong Soo Park;R. Vreeland;B. Cho;T. Lowenstein;M. Timofeeff;W. Rosenzweig
通讯作者：
Jong Soo Park;R. Vreeland;B. Cho;T. Lowenstein;M. Timofeeff;W. Rosenzweig