Novel spiral structured nerve guidance conduits with multichannels and inner longitudinally aligned nanofibers for peripheral nerve regeneration.
Novel spiral structured nerve guidance conduits with multichannels and inner longitudinally aligned nanofibers for peripheral nerve regeneration.
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DOI：
10.1002/jbm.b.34233
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发表时间：
2019-07
期刊：
影响因子：
--
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Shah MB;Chang W;Zhou G;Glavy JS;Cattabiani TM;Yu X
神经导引导管是自体神经移植物的人工替代物，是治疗周围神经损伤的金标准。组织工程化NGC中反复出现的挑战是优化横截面表面积以实现允许神经浸润与支持从近端到远端残端的最大轴突延伸之间的平衡。在这项研究中，我们通过研究具有由螺旋结构和多通道组成的更高横截面表面的NGC的功效来解决这个问题，再加上内部纵向排列的纳米纤维和蛋白质，以帮助神经修复临界尺寸的神经缺损。将NGCs植入15 mm长的大鼠坐骨神经损伤间隙中4周。使用一组已建立的测定法评估神经再生，包括步行轨迹分析、电生理学测试、夹捏反射评估、腓肠肌测量和组织学评估。结果表明，新的NGC设计在相对较短的恢复时间内促进神经再生方面产生了有希望的数据。新型NGC的神经再生性能上级优于具有管状结构的对照神经导管。
Nerve guidance conduits (NGCs) are artificial substitutes for autografts, which serve as the gold standard in treating peripheral nerve injury. A recurring challenge in tissue engineered NGCs is optimizing the cross-sectional surface area to achieve a balance between allowing nerve infiltration while supporting maximum axonal extension from the proximal to distal stump. In this study we address this issue by investigating the efficacy of an NGC with a higher cross-sectional surface composed of spiral structures and multi-channels, coupled with inner longitudinally aligned nanofibers and protein on aiding nerve repair in critical sized nerve defect. The NGCs were implanted into 15-mm long rat sciatic nerve injury gaps for 4 weeks. Nerve regeneration was assessed using an established set of assays, including the walking track analysis, electrophysiological testing, pinch reflex assessment, gastrocnemius muscle measurement, and histological assessment. The results indicated that the novel NGC design yielded promising data in encouraging nerve regeneration within a relatively short recovery time. The performance of the novel NGC for nerve regeneration was superior to that of the control nerve conduits with tubular structures.
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