Manipulation of bacteriophages with dielectrophoresis on carbon nanofiber nanoelectrode arrays.
Manipulation of bacteriophages with dielectrophoresis on carbon nanofiber nanoelectrode arrays.
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DOI：
10.1002/elps.201200486
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发表时间：
2013-04
期刊：
影响因子：
2.9
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Madiyar, Foram R.;Syed, Lateef U.;Culbertson, Christopher T.;Li, Jun
关键词：
这项工作描述了使用纳米结构介电电泳 (DEP) 装置有效操纵噬菌体病毒颗粒。 DEP 的非均匀电场是通过利用由垂直排列的碳纳米纤维 (VACNF) 制成的纳米电极阵列 (NEA) 与集成在微流体通道中的“点盖”配置的宏观氧化铟锡电极来创建的。已经系统地研究了病毒颗粒的捕获与流速、正弦交流频率、峰峰值电压和病毒浓度的关系。所有条件下的 DEP 捕获都是可逆的，当电压关闭时，捕获的病毒颗粒会立即释放。在低病毒浓度（8.9×104 pfu·ml−1）下，DEP捕获效率可达60%。病毒颗粒在隔离的纳米电极尖端处被单独捕获，并随时间线性累积。由于尺寸相当，使用这种基于 NEA 的 DEP 设备捕获病毒颗粒比捕获较大的细菌细胞更有效。该技术可潜在地用作微流控芯片中的快速样品制备模块，以捕获、分离和浓缩便携式检测系统中少量稀释溶液中的病毒和其他生物颗粒，以供现场应用。
This work describes efficient manipulation of bacteriophage virus particles using a nanostructured dielectrophoresis (DEP) device. The non-uniform electric field for DEP is created by utilizing a nanoelectrode array (NEA) made of vertically aligned carbon nanofibers (VACNFs) versus a macroscopic indium tin oxide electrode in a “points-and-lid” configuration integrated in a microfluidic channel. The capture of the virus particles has been systematically investigated versus the flow velocity, sinusoidal AC frequency, peak-to-peak voltage, and virus concentration. The DEP capture at all conditions is reversible and the captured virus particles are released immediately when the voltage is turned off. At the low virus concentration (8.9×104 pfu·ml−1), the DEP capture efficiency up to 60% can be obtained. The virus particles are individually captured at isolated nanoelectrode tips and accumulate linearly with time. Due to the comparable size, it is more effective to capture virus particles than larger bacterial cells with such NEA based DEP devices. This technique can be potentially utilized as a fast sample preparation module in a microfluidic chip to capture, separate, and concentrate viruses and other biological particles in small volumes of dilute solutions in a portable detection system for field applications.
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影响因子：
2.9
作者：
Ermolina, Irina;Milner, Joel;Morgan, Hywel
通讯作者：
Morgan, Hywel
影响因子：
2
作者：
Hughes, MP;Morgan, H;Rixon, FJ
通讯作者：
Rixon, FJ
影响因子：
3.4
作者：
Suehiro, J;Yatsunami, R;Hara, M
通讯作者：
Hara, M
影响因子：
2.9
作者：
Arumugam, Prabhu U.;Chen, Hua;Li, Jun
通讯作者：
Li, Jun
DOI：
10.1016/s0304-4165(98)00058-0
发表时间：
1998-09-16
影响因子：
3
作者：
Hughes, MP;Morgan, H;Pethig, R
通讯作者：
Pethig, R