Genetic and antigenic characterisation of serotype A FMD viruses from East Africa to select new vaccine strains.
Genetic and antigenic characterisation of serotype A FMD viruses from East Africa to select new vaccine strains.
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DOI：
10.1016/j.vaccine.2014.08.033
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发表时间：
2014-10-07
期刊：
影响因子：
5.5
通讯作者：
Mahapatra, Mana
中科院分区：
文献类型：
--
作者：
Bari, Fufa D.;Parida, Satya;Tekleghiorghis, Tesfaalem;Dekker, Aldo;Sangula, Abraham;Reeve, Richard;Haydon, Daniel T.;Paton, David J.;Mahapatra, Mana
关键词：
可以通过最近流行的病毒的抗原和遗传特征来选择针对流行国家新出现的口蹄疫病毒（FMDV）暴发的疫苗株。 1998 年至 2012 年间，从中非、东非和北非国家分离出总共 56 种 A 血清型口蹄疫病毒，通过使用 3 种现有疫苗株和 4 种候选疫苗株的抗血清进行病毒中和试验，对抗原进行了表征，并通过对完整衣壳序列数据进行了遗传学表征。对衣壳序列数据的贝叶斯分析显示，这些病毒属于非洲或亚洲拓扑型，并将非洲拓扑型病毒细分为四种基因型（基因型 I、II、IV 和 VII）。现有疫苗株的交叉反应性最低（仅 5.4-46.4% 的采样病毒具有良好的匹配性）。针对 A-EA-2007、A-EA-1981 和 A-EA-1984 病毒的三种牛抗血清对超过 85% 的流行病毒表现出广泛的交叉中和作用。在这三种疫苗中，A-EA-2007 是最好的，显示出超过 90% 的体外交叉保护，并且是本研究中使用的疫苗株中最新的。因此，它在抗原上看来适合作为疫苗株用于该地区的口蹄疫控制计划。
Vaccine strain selection for emerging foot-and-mouth disease virus (FMDV) outbreaks in enzootic countries can be addressed through antigenic and genetic characterisation of recently circulating viruses. A total of 56 serotype A FMDVs isolated between 1998 and 2012, from Central, East and North African countries were characterised antigenically by virus neutralisation test using antisera to three existing and four candidate vaccine strains and, genetically by characterising the full capsid sequence data. A Bayesian analysis of the capsid sequence data revealed the viruses to be of either African or Asian topotypes with subdivision of the African topotype viruses into four genotypes (Genotypes I, II, IV and VII). The existing vaccine strains were found to be least cross-reactive (good matches observed for only 5.4–46.4% of the sampled viruses). Three bovine antisera, raised against A-EA-2007, A-EA-1981 and A-EA-1984 viruses, exhibited broad cross-neutralisation, towards more than 85% of the circulating viruses. Of the three vaccines, A-EA-2007 was the best showing more than 90% in-vitro cross-protection, as well as being the most recent amongst the vaccine strains used in this study. It therefore appears antigenically suitable as a vaccine strain to be used in the region in FMD control programmes.
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影响因子：
2.7
作者：
Ayelet, G.;Soressa, M.;Asmare, K.
通讯作者：
Asmare, K.
影响因子：
11.8
作者：
Ayelet G;Mahapatra M;Gelaye E;Egziabher BG;Rufeal T;Sahle M;Ferris NP;Wadsworth J;Hutchings GH;Knowles NJ
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Knowles NJ
影响因子：
3.3
作者：
Mahapatra, M.;Seki, C.;Paton, D. J.
通讯作者：
Paton, D. J.
影响因子：
3.8
作者：
BOLWELL, C;CLARKE, BE;ROWLANDS, DJ
通讯作者：
ROWLANDS, DJ
影响因子：
5.5
作者：
Brehm, K. E.;Kumar, N.;Haas, B.
通讯作者：
Haas, B.