Spectrum-Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Yangyin Tongnao Prescription.
Spectrum-Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Yangyin Tongnao Prescription.
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养阴通脑方HPLC指纹图谱与抗氧化活性的谱效关系
DOI：
10.1155/2021/6650366
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发表时间：
2021
影响因子：
2.6
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Yu L;Zhang Y;Zhao X;He Y;Wan H;Wan H;Yang J
养阴通脑方是一种传统的中药方剂，具有抗氧化作用，主要用于治疗心脑血管疾病。然而，与其抗氧化活性相关的化合物仍然未知。采用高效液相色谱法（HPLC）对不同提取条件下的YYTN提取物进行指纹图谱分析，以确定所有样品的共有峰。采用2，2-二苯基-1-苦基肼（DPPH）自由基清除活性和铁还原抗氧化能力（FRAP）测定方法，对提取物的抗氧化活性进行评价。采用Pearson积差相关系数（PPMCC）和多元线性回归分析（MLRA），对YYTN的HPLC指纹图谱与其抗氧化活性之间的谱效关系进行分析。结果表明，指纹图谱中的5、6、13、15和24峰与抗氧化活性密切相关。从样品中鉴定出5个峰：香草酸（P5）、葛根素（P7）、阿魏酸（P13）、大豆苷元（P21）和芒柄花素（P23）。本研究成功建立了养阴汤抗氧化活性与HPLC指纹图谱间的谱效关系，为建立色谱法与抗氧化活性相结合的质量标准提供了通用方法。
Yangyin Tongnao (YYTN) prescription is used as a traditional Chinese herbal formula, and it has antioxidant activity that mainly contributes in the treatment of cardiovascular and cerebrovascular diseases. However, the compounds related to its antioxidant activity are still unknown. In the present study, the fingerprints of YYTN extracts under different extraction conditions were obtained by high performance liquid chromatography (HPLC) to identify the common peaks to all the samples processed. A 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and ferric reducing antioxidant power (FRAP) assay were carried out to evaluate the antioxidant activity of the extracts. Spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN was assessed by Pearson product-moment correlation coefficient (PPMCC) and multiple linear regression analysis (MLRA). The results showed that peaks 5, 6, 13, 15, and 24 of the fingerprints were closely connected to antioxidant activity. Five peaks were identified: vanillic acid (P5), puerarin (P7), ferulic acid (P13), daidzein (P21), and formononetin (P23). Our study successfully established the spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN, which provided a general method for establishing quality standards with a combination of chromatography and antioxidant activity.
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