Characterization of multiple bistratified retinal ganglion cells in a purkinje cell protein 2-Cre transgenic mouse line.
Characterization of multiple bistratified retinal ganglion cells in a purkinje cell protein 2-Cre transgenic mouse line.
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DOI：
10.1002/cne.23279
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发表时间：
2013-06-15
影响因子：
2.5
通讯作者：
Pan, Zhuo-Hua
中科院分区：
文献类型：
--
作者：
Ivanova, Elena;Lee, Patrick;Pan, Zhuo-Hua
关键词：
视网膜神经节细胞分为多个类别，包括多种类型的双层神经节细胞（BGC）。最近使用的转基因小鼠品系与特定类型的神经节细胞，标记的荧光标记物，促进了形态学和生理学研究的BGC，特别是方向选择性BGC。使用转基因动物最重要的好处是能够进行体内基因操作。特别是，Cre/LoxP重组系统已成为一个强大的工具，允许基因缺失，过表达，和异位表达的细胞类型特异性和时间控制的方式。该工具的关键是具有Cre重组酶的细胞或组织类型特异性表达的Cre小鼠系的可用性。在这项研究中，我们的特点是铬阳性视网膜神经节细胞的PCP 2（浦肯野细胞蛋白2）-Cre小鼠线。我们发现所有的Cre阳性视网膜神经节细胞都是BGC。根据形态学标准，我们确定它们可以分为五种类型。这些类型的开和关树突的胆碱能带外分层和不同的方向选择性神经节细胞（DSGCs）的形态。这些细胞对Brn-3b呈阴性，对钙视网膜蛋白和CART视网膜标记物呈阳性。其余两种类型被确定为推定的开-关和开-DSGCs。该Cre小鼠系可用于进一步研究小鼠BGC的分子和功能特性。
Retinal ganglion cells are categorized into multiple classes, including multiple types of bistratified ganglion cells (BGCs). The recent use of transgenic mouse lines with specific type(s) of ganglion cells that are labeled by fluorescent markers has facilitated the morphological and physiological studies of BGCs, particularly the directional-selective BGCs. The most important benefit from using transgenic animals is the capability to perform in vivo gene manipulation. In particular, the Cre/LoxP recombination system has become a powerful tool, allowing gene deletion, over-expression, and ectopic expression in a cell type-specific and temporally controlled fashion. The key to this tool is the availability of Cre mouse lines with cell or tissue type-specific expression of Cre recombinase. In this study, we characterized the Cre-positive retinal ganglion cells in a PCP2 (Purkinje cell protein 2)-cre mouse line. We found that all of the Cre-positive retinal ganglion cells were BGCs. Based on morphological criteria, we determined that they can be grouped into five types. The On- and Off-dendrites of three of these types stratified outside of the cholinergic bands and differed from directional selective ganglion cells (DSGCs) morphologically. These cells were negative for Brn-3b and positive for both calretinin and CART retina markers. The remaining two types were identified as putative On-Off and On-DSGCs. This Cre mouse line could be useful for further studies of the molecular and functional properties of BGCs in mice.
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影响因子：
3.3
作者：
Ivanova, E.;Hwang, G. -S.;Pan, Z. -H.
通讯作者：
Pan, Z. -H.
DOI：
10.1523/jneurosci.0907-11.2011
发表时间：
2011-05-25
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Kay JN;De la Huerta I;Kim IJ;Zhang Y;Yamagata M;Chu MW;Meister M;Sanes JR
通讯作者：
Sanes JR
影响因子：
2.5
作者：
FAMIGLIETTI, EV
通讯作者：
FAMIGLIETTI, EV
影响因子：
2.5
作者：
DOI, M;UJI, Y;YAMAMURA, H
通讯作者：
YAMAMURA, H
影响因子：
2.5
作者：
Hoshi, Hideo;Tian, Lian-Ming;Massey, Stephen C.;Mills, Stephen L.
通讯作者：
Mills, Stephen L.