Analysis of QTLs and Candidate Genes for Tassel Symptoms in Maize Infected with Sporisorium reilianum.
Analysis of QTLs and Candidate Genes for Tassel Symptoms in Maize Infected with Sporisorium reilianum.
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DOI：
10.3390/ijms232214416
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发表时间：
2022-11-20
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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热黑穗病是由玉米丝黑穗病菌引起的一种土传真菌病害，影响雌雄雄穗的发育。我们前期的研究发现，感染玉米丝黑穗病菌的玉米雄穗症状在具有四平头血统的自交系中存在显著差异，并且在多个地点随时间推移表现出稳定的遗传。细胞学分析表明，黄早4号、京7号和昌7 -2三个典型自交系在VT期的细胞组织结构存在显著差异。利用3个典型自交系构建的2个F2群体，对控制玉米穗部感染丝黑穗病菌不同症状的QTL进行定位。采用BSA（bulked segregation analysis）方法构建了基于典型雄穗症状的混合基因库。利用覆盖玉米全基因组的869个SSR标记，对玉米穗部感染丝黑穗病菌后不同症状的QTL进行了检测。利用双亲间的多态性标记对混合基因库进行筛选。在上述标记附近添加额外的SSR标记，以检测F2群体中部分单株的基因型。黄早4号和京7号雄穗性状的QTL位于标记umc 1590和bnlg 1598之间的bin 1.06区域，可解释21.12%的表型变异，加性效应为0.6524。控制京7和昌7 -2雄穗症状的QTL位于bin 2.07区域，介于标记umc 1042和bnlg 1335之间，可解释11.26%的表型变异，加性效应为0.4355。两个候选基因（ZmABP 2和Zm 00001 D 006403）的无标记定量蛋白质组测序的联合分析确定。
Heat smut is a fungal soil-borne disease caused by Sporisorium reilianum, and affects the development of male and female tassels. Our previous research found that the tassel symptoms in maize infected with Sporisorium reilianum significantly differed in inbred lines with Sipingtou blood, and exhibited stable heredity over time at multiple locations. In this study, cytological analysis demonstrated that the cellular organization structures of three typical inbred lines (Huangzao4, Jing7, and Chang7-2) showed significant discrepancies at the VT stage. QTLs that control the different symptoms of maize tassels infected with Sporisorium reilianum were located in two F2 populations, which were constructed using three typical inbred lines. The BSA (bulked segregation analysis) method was used to construct mixed gene pools based on typical tassel symptoms. The QTLs of different symptoms of maize tassels infected with Sporisorium reilianum were detected with 869 SSR markers covering the whole maize genome. The mixed gene pools were screened with polymorphic markers between the parents. Additional SSR markers were added near the above marker to detect genotypes in partially single plants in F2 populations. The QTL controlling tassel symptoms in the Huangzao4 and Jing7 lines was located on the bin 1.06 region, between the markers of umc1590 and bnlg1598, and explained 21.12% of the phenotypic variation with an additive effect of 0.6524. The QTL controlling the tassel symptoms of the Jing7 and Chang7-2 lines was located on the bin 2.07 region, between the markers of umc1042 and bnlg1335, and explained 11.26% phenotypic variation with an additive effect of 0.4355. Two candidate genes (ZmABP2 and Zm00001D006403) were identified by a conjoint analysis of label-free quantification proteome sequencings.
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