Caenorhabditis elegans deficiency mapping.
Caenorhabditis elegans deficiency mapping.
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秀丽隐杆线虫缺乏图谱。
DOI：
10.1093/genetics/108.2.331
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Herman,RK
中科院分区：
文献类型：
--
作者：
Sigurdson,DC;Spanier,GJ;Herman,RK
用六种方案鉴定了线虫X射线处理后80个独立的连锁群（LG）II隐性致死缺陷。互补测试之间的缺陷和甲基磺酸乙酯诱导的隐性可见，致死和不育突变和不同的缺陷之间被用来表征的缺陷的程度。因此，缺陷端点有助于订购36个网站内的一个区域，代表约一半的基因座LG II和延伸超过约5个地图单位。通过对少量缺陷的互补测试，可以将该区域发生的新突变分配给图谱的特定片段;这有助于将单位点突变分配给特定基因，如我们所示。5个精子缺陷和5个卵母细胞缺陷LG II不育突变体进行了鉴定和定位。某些缺陷的缺陷互补试验使我们能够表明，在两个基因座的无效突变的表型由可见的等位基因代表是野生型，在第三个基因座的无效突变赋予可见的表型。LG II的一个片段长约12个图位单位，基本上没有识别的基因座，似乎非常有利于交换。
Six schemes were used to identify 80 independent recessive lethal deficiencies of linkage group (LG) II following X-ray treatment of the nematodeCaenorhabditis elegans. Complementation tests between the deficiencies and ethyl methanesulfonate-induced recessive visible, lethal and sterile mutations and between different deficiencies were used to characterize the extents of the deficiencies. Deficiency endpoints thus helped to order 36 sites within a region representing about half of the loci on LG II and extending over about 5 map units. New mutations occurring in this region can be assigned to particular segments of the map by complementation tests against a small number of deficiencies; this facilitates the assignment of single-site mutations to particular genes, as we illustrate. Five sperm-defective and five oocyte-defective LG II sterile mutants were identified and mapped. Certain deficiency-by-deficiency complementation tests allowed us to suggest that the phenotypes of null mutations at two loci represented by visible alleles are wild type and that null mutations at a third locus confer a visible phenotype. A segment of LG II that is about 12 map units long and largely devoid of identified loci seems to be greatly favored for crossing over.
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影响因子：
2.7
作者：
CASSADA, R;ISNENGHI, E;VONEHRENSTEIN, G
通讯作者：
VONEHRENSTEIN, G
影响因子：
2.7
作者：
WOOD, WB;HECHT, R;HIRSH, D
通讯作者：
HIRSH, D
unc-93(e1500)：秀丽隐杆线虫的行为突变体，定义了具有野生型无效表型的基因。
影响因子：
3.3
作者：
Greenwald,IS;Horvitz,HR
通讯作者：
Horvitz,HR
秀丽隐杆线虫 unc-22 IV 区域的必需基因和缺陷。
影响因子：
3.3
作者：
T. Rogalski;D. Moerman;D. Baillie
通讯作者：
D. Baillie
影响因子：
2.5
作者：
LEWIS, JA;HODGKIN, JA
通讯作者：
HODGKIN, JA