Expression of Glutamate and GABA during the Process of Rat Retinal Synaptic Plasticity Induced by Acute High Intraocular Pressure.
Expression of Glutamate and GABA during the Process of Rat Retinal Synaptic Plasticity Induced by Acute High Intraocular Pressure.
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急性高眼压致大鼠视网膜突触可塑性过程中谷氨酸和GABA的表达
DOI：
10.1267/ahc.12029
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发表时间：
2013-02-28
影响因子：
2.4
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Zhou L;Huang J;Wang H;Luo J;Zeng L;Xiong K;Chen D
急性高眼压（Acute high intraocular pressure，HIOP）可引起视网膜突触的可塑性改变，突触前标志物突触体素（synaptophysin，SYN）的表达从内丛状层到外丛状层具有明显的时空变化。我们确定了视网膜中参与这一过程的神经递质类型，并确定了这些神经递质对HIOP诱导的反应。前房注射生理盐水建立急性高眼压模型。我们发现，谷氨酸阳性细胞的数量增加，从内部到外部的部分的视网膜（从神经节细胞层的内核层的外核层）后HIOP，这是类似的SYN表达的时空模式（内部到外部）后HIOP。GABA在视网膜的分布和强度在伤后不同时间无明显变化，与SYN表达无直接相关性。我们的结果表明，兴奋性神经递质谷氨酸可能参与了急性高眼压后视网膜突触的可塑性过程，但没有证据表明抑制性神经递质GABA的作用。
Acute high intraocular pressure (HIOP) can induce plastic changes of retinal synapses during which the expression of the presynaptic marker synaptophysin (SYN) has a distinct spatiotemporal pattern from the inner plexiform layer to the outer plexiform layer. We identified the types of neurotransmitters in the retina that participated in this process and determined the response of these neurotransmitters to HIOP induction. The model of acute HIOP was established by injecting normal saline into the anterior chamber of the rat eye. We found that the number of glutamate-positive cells increased successively from the inner part to the outer part of the retina (from the ganglion cell layer to the inner nuclear layer to the outer nuclear layer) after HIOP, which was similar to the spatiotemporal pattern of SYN expression (internally to externally) following HIOP. However, the distribution and intensity of GABA immunoreactivity in the retina did not change significantly at different survival time post injury and had no direct correlation with SYN expression. Our results suggested that the excitatory neurotransmitter glutamate might participate in the plastic process of retinal synapses following acute HIOP, but no evidence was found for the role of the inhibitory neurotransmitter GABA.
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影响因子：
1.9
作者：
Darwish H;Mahmood A;Schallert T;Chopp M;Therrien B
通讯作者：
Therrien B
影响因子：
4.2
作者：
Moreno, Maria Cecilia;de Zavalia, Nuria;Rosenstein, Ruth E.
通讯作者：
Rosenstein, Ruth E.
影响因子：
2.9
作者：
Pereno, German I.;Beltramino, Carlos A.
通讯作者：
Beltramino, Carlos A.
影响因子：
--
作者：
Maragakis, NJ;Rothstein, JD
通讯作者：
Rothstein, JD
影响因子：
16.2
作者：
Kwon SE;Chapman ER
通讯作者：
Chapman ER