A suspended layer additive manufacturing approach to the bioprinting of tri-layered skin equivalents.
A suspended layer additive manufacturing approach to the bioprinting of tri-layered skin equivalents.
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DOI：
10.1063/5.0061361
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发表时间：
2021-12
影响因子：
6
通讯作者：
Grover LM
中科院分区：
文献类型：
--
作者：
Moakes RJA;Senior JJ;Robinson TE;Chipara M;Atansov A;Naylor A;Metcalfe AD;Smith AM;Grover LM
皮肤结构复杂，主要由三层组成（表皮、真皮层和真皮）。广泛的创伤可能导致这些结构的丧失和修复不良，从长远来看，形成疤痕组织和相关的功能下降。虽然存在许多皮肤替代品，但没有解决方案能够再现天然皮肤中存在的化学，机械和生物作用。这项研究报告了使用悬浮层增材制造来生产一个连续的三层植入物，它与人体皮肤非常相似。通过仔细控制生物墨水的组成，在整个打印结构中形成了梯度（化学和细胞）。模型培养表明，在21天内，细胞成分在将支撑基质重塑成与健康皮肤相当的结构方面发挥了关键作用。事实上，已经证明，即使在植入后7天，植入物的整合已经发生，脂肪组织从周围组织动员到构建物本身。因此，人们相信这些植入物可以促进愈合，从筋膜开始，向上到皮肤表面，这一机制最近被证明是深度伤口的关键。
Skin exhibits a complex structure consisting of three predominant layers (epidermis, dermis, and hypodermis). Extensive trauma may result in the loss of these structures and poor repair, in the longer term, forming scarred tissue and associated reduction in function. Although a number of skin replacements exist, there have been no solutions that recapitulate the chemical, mechanical, and biological roles that exist within native skin. This study reports the use of suspended layer additive manufacturing to produce a continuous tri-layered implant, which closely resembles human skin. Through careful control of the bioink composition, gradients (chemical and cellular) were formed throughout the printed construct. Culture of the model demonstrated that over 21 days, the cellular components played a key role in remodeling the supporting matrix into architectures comparable with those of healthy skin. Indeed, it has been demonstrated that even at seven days post-implantation, the integration of the implant had occurred, with mobilization of the adipose tissue from the surrounding tissue into the construct itself. As such, it is believed that these implants can facilitate healing, commencing from the fascia, up toward the skin surface—a mechanism recently shown to be key within deep wounds.
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