Development of the oral resistome during the first decade of life.
Development of the oral resistome during the first decade of life.
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DOI：
10.1038/s41467-023-36781-w
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发表时间：
2023-03-09
影响因子：
16.6
通讯作者：
Adler CJ
中科院分区：
文献类型：
--
作者：
Sukumar S;Wang F;Simpson CA;Willet CE;Chew T;Hughes TE;Bockmann MR;Sadsad R;Martin FE;Lydecker HW;Browne GV;Davis KM;Bui M;Martinez E;Adler CJ
抗生素的过度使用促进了抗菌素耐药性(AMR)的传播，带来了重大的健康和经济后果。基因组测序揭示了在各种微生物环境中广泛存在的抗菌素耐药基因(ARGs)。因此，对耐药库进行监测，就像很少探索的口腔微生物群一样，对于抗击AMR是必要的。在这里，我们描述了儿童口腔抵抗器的发育特征，并调查了221名双胞胎儿童(124名女性和97名男性)在出生后第一个十年的三个时间点上的龋齿作用。从530个口腔元基因组中，我们识别了309个ARG，它们显著地按年龄聚集，从婴儿时期开始检测到宿主遗传效应。我们的结果表明，随着年龄的增长，ARGs的潜在动员能力增加，因为AMR相关的移动遗传元件Tn916转座酶在较大的儿童中与更多的物种和ARGs共存。我们发现，与健康相比，龋齿中的ARGs和种类减少了。在修复牙齿时，这一趋势正好相反。在这里，我们表明儿科口腔耐药组是口腔微生物组固有的和动态的组成部分，在AMR和微生态失调的传播中具有潜在的作用。在这里，作者对人类口腔微生物组在生命的第一个十年的抗药性潜力进行了纵向遗传监测，揭示了一个因龋齿而改变的动态环境，随着儿童的成长，动员基因的潜力越来越大。
Antibiotic overuse has promoted the spread of antimicrobial resistance (AMR) with significant health and economic consequences. Genome sequencing reveals the widespread presence of antimicrobial resistance genes (ARGs) in diverse microbial environments. Hence, surveillance of resistance reservoirs, like the rarely explored oral microbiome, is necessary to combat AMR. Here, we characterise the development of the paediatric oral resistome and investigate its role in dental caries in 221 twin children (124 females and 97 males) sampled at three time points over the first decade of life. From 530 oral metagenomes, we identify 309 ARGs, which significantly cluster by age, with host genetic effects detected from infancy onwards. Our results suggest potential mobilisation of ARGs increases with age as the AMR associated mobile genetic element, Tn916 transposase was co-located with more species and ARGs in older children. We find a depletion of ARGs and species in dental caries compared to health. This trend reverses in restored teeth. Here we show the paediatric oral resistome is an inherent and dynamic component of the oral microbiome, with a potential role in transmission of AMR and dysbiosis. Here, the authors provide a longitudinal genetic surveillance of the antimicrobial resistance potential of the human oral microbiome in the first decade of life, revealing a dynamic environment altered by tooth decay with the increasing potential to mobilize genes as children grow.
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影响因子：
7
作者：
Clark SE
通讯作者：
Clark SE
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
11
作者：
Dzidic, Majda;Collado, Maria C.;Mira, Alex
通讯作者：
Mira, Alex
影响因子：
4.6
作者：
Freire, Marcelo;Moustafa, Ahmed;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T