Functional collagen fiber architecture of the pulmonary heart valve cusp.
Functional collagen fiber architecture of the pulmonary heart valve cusp.
复制标题
DOI：
10.1016/j.athoracsur.2008.12.049
复制
发表时间：
2009-04
影响因子：
4.6
通讯作者：
Sacks, Michael S.
中科院分区：
文献类型：
--
作者：
Joyce, Erinn M.;Liao, Jun;Schoen, Frederick J.;Mayer, John E., Jr.;Sacks, Michael S.
肺动脉瓣（PV）缺陷发生在各种形式的先天性心脏病中。关于肺静脉胶原纤维结构的定量信息，特别是其对舒张力的反应，对于肺静脉修复和置换方法的设计和功能评估是必要的。对于新型组织工程PV尤其如此，其依赖于广泛的体内重塑以实现长期功能。在0 - 90 mmHg跨瓣压下，将猪PV和主动脉瓣（AV）固定为0 - 90 mmHg跨瓣压。从根部解剖后，进行小角光散射（SALS）测量，以量化胶原纤维结构和整个尖瓣上施加的跨瓣压力增加时的变化。还进行了组织形态学测量，以评估瓣尖层厚度随压力的变化。虽然PV和AV显示出预期的结构相似性，但它们也存在重要的功能相关差异。在未加载状态下，AV尖在纤维排列方面表现出显著的区域变化，而PV令人惊讶地均匀。此外，与PV相比，AV显示出胶原纤维排列随施加的跨瓣压力的显著更大变化。总体而言，AV胶原纤维网络表现出对施加的跨瓣压力的更大响应能力。压握幅度降低是两种瓣膜中胶原纤维定向程度改善的主要机制。本研究阐明了PV和AV之间的主要相似性和差异。虽然强调了PV如何能够作为患病主动脉瓣的适当置换物，但观察到的结构差异也可能表明PV完全复制AV的能力有限。此外，定量数据从这项研究的PV功能架构将有利于组织工程PV的发展，通过定义关键的纤维结构特征。
Defects in the pulmonary valve (PV) occur in a variety of forms of congenital heart diseases. Quantitative information on PV collagen fiber architecture, and particularly its response to diastolic forces, is necessary for the design and functional assessment of approaches for PV repair and replacement. This is especially the case for novel tissue engineered PV, which rely on extensive in-vivo remodeling for long-term function. Porcine PV and aortic valves (AV) were fixed 0 to 90 mmHg transvalvular under a 0 to 90 mmHg transvalvular pressure. After dissection from the root, small angle light scattering (SALS) measurements were conducted to quantify the collagen fiber architecture and changes with increasing applied transvalvular pressure over the entire cusp. Histomorphologic measurements were also performed to assess changes in cuspal layer thickness with pressure. While the PV and AV displayed anticipated structural similarities, they also presented important functionally related differences. In the unloaded state, the AV cusp demonstrated substantial regional variations in fiber alignment, whereas the PV was surprisingly uniform. Further, the AV demonstrated substantially larger changes in collagen fiber alignment with applied transvalvular pressure compared to the PV. Overall, the AV collagen fiber network demonstrated greater ability to respond to applied transvalvular pressure. A decrease in crimp amplitude was the predominant mechanism for improvement in the degree of orientation of the collagen fibers in both valves. This study clarified the major similarities and differences between the PV and AV. While underscoring how the PV can serve as an appropriate replacement of the diseased aortic valve, the observed structural differences may also indicate limits to the PV's ability to fully duplicate the AV. Moreover, quantitative data from this study on PV functional architecture will benefit development of tissue engineered PV by defining the critical fiber architectural characteristics.
登录
查看更多内容
影响因子：
3.8
作者：
Iyengar, AKS;Sugimoto, H;Sacks, MS
通讯作者：
Sacks, MS
DOI：
10.1016/s0735-1097(00)00834-2
发表时间：
2000-10-01
影响因子：
24
作者：
Hammermeister, K;Sethi, GK;Rahimtoola, SH
通讯作者：
Rahimtoola, SH
DOI：
10.1016/s0022-5223(94)70145-8
发表时间：
1994-12-01
影响因子：
6
作者：
LOCKIE, KJ;FISHER, J;WATTERSON, K
通讯作者：
WATTERSON, K
影响因子：
3.4
作者：
Stradins, P;Lacis, R;Kasyanov, V
通讯作者：
Kasyanov, V
影响因子：
3.8
作者：
Sacks, MS;Smith, DB;Hiester, ED
通讯作者：
Hiester, ED