Microgravity simulation by diamagnetic levitation: effects of a strong gradient magnetic field on the transcriptional profile of Drosophila melanogaster.
Microgravity simulation by diamagnetic levitation: effects of a strong gradient magnetic field on the transcriptional profile of Drosophila melanogaster.
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DOI：
10.1186/1471-2164-13-52
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发表时间：
2012-02-01
期刊：
影响因子：
4.4
通讯作者：
Marco R
中科院分区：
文献类型：
--
作者：
Herranz R;Larkin OJ;Dijkstra CE;Hill RJ;Anthony P;Davey MR;Eaves L;van Loon JJ;Medina FJ;Marco R
许多生物系统对重力的存在或不存在作出反应。由于在空间进行的实验费用昂贵，而且只能偶尔进行，因此使用了地面模拟技术来研究对失重的生物反应。高梯度磁场可用于悬浮生物有机体，使其净重为零。我们用超导磁体来评估抗磁悬浮对果蝇D。黑腹果蝇在悬浮实验中进行了长达22天的连续试验。我们进行了比较，在微重力模拟的另一个范例，随机定位机（RPM）的类似实验的结果。我们观察到果蝇从胚胎到成虫的发育延迟。微阵列分析表明，在总体基因表达的变化，从幼虫开发的抗磁悬浮，也在模拟超重力条件下。免疫、应激和温度反应基因的表达发生了显著变化。例如，几种热休克蛋白受到影响。我们还发现，16.5特斯拉的强磁场对这些基因的表达有显著影响，与磁致悬浮和超重力相关的影响无关。抗磁悬浮可用于模拟改变的有效重力环境，其中基因表达在包括不同年龄和性别的不同黑腹果蝇种群中进行差异调节。暴露于磁场本身诱导了类似的，但较弱的基因表达变化。
Many biological systems respond to the presence or absence of gravity. Since experiments performed in space are expensive and can only be undertaken infrequently, Earth-based simulation techniques are used to investigate the biological response to weightlessness. A high gradient magnetic field can be used to levitate a biological organism so that its net weight is zero. We have used a superconducting magnet to assess the effect of diamagnetic levitation on the fruit fly D. melanogaster in levitation experiments that proceeded for up to 22 consecutive days. We have compared the results with those of similar experiments performed in another paradigm for microgravity simulation, the Random Positioning Machine (RPM). We observed a delay in the development of the fruit flies from embryo to adult. Microarray analysis indicated changes in overall gene expression of imagoes that developed from larvae under diamagnetic levitation, and also under simulated hypergravity conditions. Significant changes were observed in the expression of immune-, stress-, and temperature-response genes. For example, several heat shock proteins were affected. We also found that a strong magnetic field, of 16.5 Tesla, had a significant effect on the expression of these genes, independent of the effects associated with magnetically-induced levitation and hypergravity. Diamagnetic levitation can be used to simulate an altered effective gravity environment in which gene expression is tuned differentially in diverse Drosophila melanogaster populations including those of different age and gender. Exposure to the magnetic field per se induced similar, but weaker, changes in gene expression.
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影响因子：
4.9
作者：
Herranz, Raul;Benguria, Alberto;Marco, Roberto
通讯作者：
Marco, Roberto
影响因子：
5.8
作者：
Eichler, GS;Huang, S;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
3.8
作者：
Coleman, Chasity B.;Gonzalez-Villalobos, Romer A.;Hammond, Timothy G.
通讯作者：
Hammond, Timothy G.
影响因子：
--
作者：
CHURCH, RB;ROBERTSON, FW
通讯作者：
ROBERTSON, FW
影响因子：
4.1
作者：
Benguria, A;Grande, E;Marco, R
通讯作者：
Marco, R