Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge.
Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge.
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从南大西洋中脊热液喷口沉积物中分离出来的石油降解菌群的重建和评估
DOI：
10.1038/s41598-021-80991-5
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发表时间：
2021-01-14
影响因子：
4.6
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Ma M;Zheng L;Yin X;Gao W;Han B;Li Q;Zhu A;Chen H;Yang H
在这项研究中，从南大西洋深海热液区的两个不同地点收集了沉积物。从两个采样点的沉积物中富集的两种微生物富集菌（H7 S和H11 S）均能有效降解石油烃。采用高通量测序法分析细菌多样性。采用气相色谱-质谱联用仪和重量分析法评价其石油降解能力。深海热液区富集培养物中优势石油降解菌为假单胞菌属、硝酸还原菌属、不动杆菌属和短单胞菌属。经过14天的降解实验，富集培养物H11 S，这是附近的热液喷口，表现出更高的降解效率为烷烃（95%）和多环芳烃（88%）比富集培养物H7 S。富集培养物H11 S对降植烷和植烷的降解率分别达到90%和91%。菌株H11 S-31和假单胞菌菌株H11 S-28以95：4：1密度比用于构建高效石油降解菌群H。经过3天的降解实验，H菌对总烷烃和多环芳烃的降解率分别达到74.2%和65.7%。生物标志物降植烷和高分子量多环芳烃的降解率分别达到84.5%和80.48%。研究结果表明，深海热液区微生物是潜在的石油烃降解资源。人工构建的H菌群具有高效的石油降解能力，在石油污染生物修复中具有重要的应用前景。
In this study, sediments were collected from two different sites in the deep-sea hydrothermal region of the South Atlantic Ocean. Two microbial enrichment cultures (H7S and H11S), which were enriched from the sediments collected at two sample sites, could effectively degrade petroleum hydrocarbons. The bacterial diversity was analyzed by high-throughput sequencing method. The petroleum degradation ability were evaluated by gas chromatography–mass spectrometry and gravimetric analysis. We found that the dominant oil-degrading bacteria of enrichment cultures from the deep-sea hydrothermal area belonged to the generaPseudomonas,Nitratireductor,Acinetobacter, andBrevundimonas. After a 14-day degradation experiment, the enrichment culture H11S, which was obtained near a hydrothermal vent, exhibited a higher degradation efficiency for alkanes (95%) and polycyclic aromatic hydrocarbons (88%) than the enrichment culture H7S. Interestingly, pristane and phytane as biomarkers were degraded up to 90% and 91% respectively by the enrichment culture H11S, and six culturable oil-degrading bacterial strains were isolated.Acinetobacter juniistrain H11S-25,Nitratireductorsp. strain H11S-31 andPseudomonassp. strain H11S-28 were used at a density ratio of 95:4:1 to construct high-efficiency oil-degrading consortium H. After a three-day biodegradation experiment, consortium H showed high degradation efficiencies of 74.2% and 65.7% for total alkanes and PAHs, respectively. The degradation efficiency of biomarkers such as pristane and high-molecular-weight polycyclic aromatic hydrocarbons (such as CHR) reached 84.5% and 80.48%, respectively. The findings of this study indicate that the microorganisms in the deep-sea hydrothermal area are potential resources for degrading petroleum hydrocarbons. Consortium H, which was artificially constructed, showed a highly efficient oil-degrading capacity and has significant application prospects in oil pollution bioremediation.
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影响因子：
0.8
作者：
Emmanuel, E. S. Challaraj;Priya, S. Sree;George, Sona
通讯作者：
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影响因子：
2.2
作者：
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影响因子：
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作者：
La Duc, Myron T.;Benardini, James N.;Venkateswaran, Kasthuri
通讯作者：
Venkateswaran, Kasthuri
影响因子：
2.8
作者：
Luz, AP;Pellizari, VH;Greer, CW
通讯作者：
Greer, CW